Research Paper | Published:

“Primatization” of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4

Bio/Technologyvolume 10pages14551460 (1992) | Download Citation

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Abstract

Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%–98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys immunized with human CD4 antigen and a human/monkey chimeric anti-CD4 antibody with 91–92% homology to human immunoglobulin framework regions was cloned and expressed. The antibody has an apparent affinity of 3.2 × 10−11 M and exhibits potent immunosuppressive properties in vitro.

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Author notes

  1. Roland Newman: Corresponding author.

Affiliations

  1. IDEC Pharmaceuticals Corporation, 11099 North Torrey Pines Rd., La Jolla, CA, 92037

    • Roland Newman
    • , James Alberts
    • , Darrell Anderson
    • , Kristin Carner
    • , Cheryl Heard
    • , Frank Norton
    • , Ron Raab
    • , Mitchell Reff
    • , Steve Shuey
    •  & Nabil Hanna

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https://doi.org/10.1038/nbt1192-1455