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“Primatization” of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4


Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%–98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys immunized with human CD4 antigen and a human/monkey chimeric anti-CD4 antibody with 91–92% homology to human immunoglobulin framework regions was cloned and expressed. The antibody has an apparent affinity of 3.2 × 10−11 M and exhibits potent immunosuppressive properties in vitro.

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Newman, R., Alberts, J., Anderson, D. et al. “Primatization” of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4. Nat Biotechnol 10, 1455–1460 (1992).

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