Research Paper | Published:

Rescuing Transgene Expression by Co-Integration

Bio/Technologyvolume 10pages14501454 (1992) | Download Citation



To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep β-lactoglobulin gene with two poorly expressed β-lactoglobulin-derived genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. “Rescuing” transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.

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  1. A. J. Clark: Corresponding author.


  1. AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, EH25 9PS, Scotland

    • A. J. Clark
    • , A. Cowper
    • , R. Wallace
    •  & J. P. Simons
  2. Pharmaceutical Proteins Ltd, Centre for Genome Research, King's Buildings, West Mains Road, Edinburgh, EH9 3JQ, Scotland

    • G. Wright


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