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The Removal of Adventitious Viruses and Virus-Infected Cells Using a Cellular Adsorbent: A Feasibility Study

Bio/Technology volume 6, pages 1330–1333 (1988)Cite this article

Abstract

The presence of adventitious viruses in blood products for transfusion poses serious risks. Using cell surface receptors as affinity ligands, we have developed a novel approach to remove both free virions and virus-infected cells with minimal protein loss. The affinity adsorbent was prepared by immobilizing cells or cell membranes that bear the complementary receptors for the target viruses on inert polymer support surfaces, followed by chemically cross-linking the cells with glutaraldehyde. The stabilization process did not impede the adsorption process as measured in herpes simplex virus and human cytomegalovirus systems. Cellular adsorbents prepared in this manner have the advantages of high binding specificity, broad adsorption spectrum, and ease of preparation.

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Author notes

  1. Henry Y. Wang: Corresponding author.

Authors and Affiliations

  1. Department of Chemical Engineering, The University of Michigan, Ann Arbor, MI, 48109

    I-Fu Tsao & Henry Y. Wang

  2. Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI, 48109

    Charles Shipman Jr.

Authors
  1. I-Fu Tsao
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  2. Charles Shipman Jr.
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  3. Henry Y. Wang
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Tsao, IF., Shipman, C. & Wang, H. The Removal of Adventitious Viruses and Virus-Infected Cells Using a Cellular Adsorbent: A Feasibility Study. Nat Biotechnol 6, 1330–1333 (1988). https://doi.org/10.1038/nbt1188-1330

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  • DOI: https://doi.org/10.1038/nbt1188-1330

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