Abstract
A new type of microcarrier, based on gelatin, which possess large cavities available for the growth of anchorage–dependent animal cells, has been prepared. Besides the increase of surface area, this semi–entrapment protects the fragile cells against mechanical damage. Cell yields as well as plating efficiencies were found to be superior to solid microcarriers.
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References
Spier, R.E.J. 1982. Recent developments in the large scale cultivation of animal cells in monolayers. Chem. Technol. Biotechnol. 32:304–312
Haynes, J. and Weissmann, C. 1983. Constitutive, long-term production of human interferons by hamster cells containing multiple copies of a cloned interferon gene. Nucleic Acid Res. 11:687–706
van Wezel, A.L. 1967. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 216:64–65
Levine, D.W., Wang, D.I.C. and Thilly, W.G. 1979. Optimization of growth surface parameters in microcarrier cell culture. Biotechnol. Bioeng 21:821–845
Nilsson, K. and Mosbach, K. 1980. Preparation of immobilized animal cells. FEBS Lett. 118:145–150
Johansson, A. and Nielsen, V. 1980. Biosilon a new microcarrier. Develop. Biol. Standard. 46:125–129
Reuveny, S., Mizrahi, A., Kotler, M. and Freeman, A. 1983. Factors affecting cell attachment, spreading, and growth on derivatized microcarriers. I. Establishment of working system and effect of the type of the amino-charged groups. Biotechnol. Bioeng. 25:469–480
Hu, W.S., Meier, J. and Wang, D.I.C. 1985. A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers. Biotechnol. Bioeng. 27:585–595
Nilsson, K., Scheirer, W., Merten, O.W., Östberg, L., Liehl, E., Katinger, H.W.D. and Mosbach, K. 1983. Entrapment of animal cells for production of monoclonal antibodies and other biomolecules. Nature 302:629–630
Nilsson, K. and Mosbach, K. 1985. Macroporous particles. Swedish Patent Application no. 8504764-5
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Nilsson, K., Buzsaky, F. & Mosbach, K. Growth of Anchorage–Dependent Cells on Macroporous Microcarriers. Nat Biotechnol 4, 989–990 (1986). https://doi.org/10.1038/nbt1186-989
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DOI: https://doi.org/10.1038/nbt1186-989
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