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Celebrating diversity and mimicking maturation

B-cell lines that hypermutate their immunoglobulin genes can be exploited to generate antigen-specific antibodies.

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Figure 1: Original Ramos or DT40 cell (upper left) expressing a parental antibody is allowed to proliferate with simultaneous somatic hypermutation, generating progeny expressing a diverse repertoire of antibodies (lower left).

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Presta, L. Celebrating diversity and mimicking maturation. Nat Biotechnol 20, 1096–1097 (2002). https://doi.org/10.1038/nbt1102-1096

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