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Human hematopoietic progenitor cell isolation based on galactose-specific cell surface binding

Nature Biotechnology volume 15, pages 1007–1011 (1997)Cite this article

Abstract

The ability to isolate functional populations of hematopoietic progenitor cells is important to the process of hematopoietic cell transplantation and to the understanding of hematopoietic cell biology in health and disease. We show that a subpopulation of human bone marrow hematopoietic cells bearing the pan-hematopoietic antigen CD34 also binds galactose-conjugated proteins. This lectin-positive sub-population represents approximately 0.1 to 0.5% of the total bone marrow cells, and contains 100% of the hematopoietic progenitor cells. The galactose-binding lectin on these cells is specific for this sugar. Additionally, highly proliferative hematopoietic progenitor cells with very primitive phenotypes, including a newly identified progenitor cell that produces multiple lineages, express this lectin.

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References

  1. Long, M.W. 1992. Blood cell cytoadhesion molecules. Exp. Hematol. 20: 288–301.

    CAS  PubMed  Google Scholar 

  2. Sutherland, D.R. and Keating, A. 1992. The CD34 antigen: structure, biology, and potential clinical applications. [Review]. J. Hematother. 1: 115–129.

    Article  CAS  PubMed  Google Scholar 

  3. Murray, L.J., Chen, B., Galy, A., Chen, S., Tushinski, R., Uchida, N. et al. 1995. Enrichment of human hematopoietic stem cell activity in the CD34+Thy-1 +Lin-subpopulation from mobilized peripheral blood. Blood 85: 368–378.

    CAS  PubMed  Google Scholar 

  4. Hardy, C.L., Matsuoka, T. and Tavassoli, M. 1991. Distribution of homing protein on hemopoietic stromal and progenitor cells. Exp. Hematol. 19: 968–972.

    CAS  PubMed  Google Scholar 

  5. Aizawa, S. and Tavassoli, M. 1987. In vitro homing of hemopoietic stem cells is mediated by a recognition system with galactosyl and mannosyl specificities. Proc. Natl. Acad. Sci. USA 84: 4485–4489.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Sullenberger, B.A., Petitt, M.S., Chong, P., Long, M.W. and Wicha, M.S. 1995. Murine granulocytic cell adhesion to bone marrow hemonectin is mediated by mannose and galactose. Blood 86: 135–140.

    Google Scholar 

  7. Berardi, A.C., Wang, A., Levine, J.D., Lopez, P. and Scadden, D.T. 1995. Functional isolation and characterization of human hematopoietic stem cells. Science 267: 104–108.

    Article  CAS  PubMed  Google Scholar 

  8. Van Epps, D.E., Bender, J., Schilling, M., Smith, S., Unverzagt, K., Law, P. and Burgess, J. 1994. Harvesting, charachterization, and culture of CD34+ cells from human bone marrow peripheral blood and cord blood. Blood Cells 20: 411–423.

    CAS  PubMed  Google Scholar 

  9. Rusten, L., Jacobsen, S., Kaalhus, J., Veilby, O., Funderud, S. and Smeland, E. 1994. Functional differences between CD38- and DR- subfractions of CD34+ bone marrow cells. Blood 84: 1473–1481.

    CAS  PubMed  Google Scholar 

  10. Nagler, A., Peacock, M., Tantoco, M., Lamons, D., Okarma, T. and Okrongly, D. 1994. Red blood cell depletion and enrichment of CD34+ hematopoietic progenitor cells from human umbilical cord blood using soybean agglutinin and CD34 immunoselection. Exp. Hematol. 22: 1134–1140.

    CAS  PubMed  Google Scholar 

  11. Lansdorp, P.M., Sutherland, H.J. and Eaves, C.J. 1990. Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow. J. Exp. Med. 172: 363–366.

    Article  CAS  PubMed  Google Scholar 

  12. Craig, W., Kay, R., Cutler, R.L. and Lansdorp, P.M. 1993. Expression of Thy-1 on human hematopoietic progenitor cells. J. Exp. Med. 177: 1331–1342.

    Article  CAS  PubMed  Google Scholar 

  13. Long, M.W., Hutchinson, R.J., Gragowski, L.L., Heffner, C.H. and Emerson, S.G. 1988. Synergistic regulation of human megakaryocyte development. J. Clin. Invest. 82: 1779–1786.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. de Wynter, E.A., Coutinho, L.H., Pei, X., Marsh, J.C., Hows, J., Luft, T., and Testa, N.G. 1995. Comparison of purity and enrichment of CD34* cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems. Stem Cells 13: 532.

    Article  Google Scholar 

  15. McNeice, I.K., Stewart, F.M., Deacon, D.M., Temeles, D.S., Zsebo, K.M., Clark, S.C. and Quesenberry, P.J. 1989. Detection of a human CFC with a high prolif-erative potential. Blood 74: 609–612.

    Google Scholar 

  16. Long, M.W., Briddell, R., Walter, A.W., Bruno, E. and Hoffman, R. 1992. Human hematopoietic stem cell adherence to cytokines and matrix molecules. J. Clin. Invest. 90: 251–255.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Carow, C.E., Hangoc, G. and Broxmeyer, H.E. 1993. Human multipotential progenitor cells have extensive replating capacity for secondary CFU-GEMM: an effect enhanced by cord blood plasma. Blood 81: 942–949.

    CAS  PubMed  Google Scholar 

  18. Huyhn, A., Dommergues, M., Izac, B., Croisille, L., Katz, A., Vainchenker, W. and Coulombel, L. 1995. Characterization of hematopoietic progenitors from human yolk sacs and embryos. Blood 86: 4474–4485.

    CAS  PubMed  Google Scholar 

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Author notes

  1. Michael W. Long: Corresponding author: (e-mail: MWLong@umich.edu).

Authors and Affiliations

  1. Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI, 48109

    George G. Pipia

  2. Department of Pediatrics, University of Michigan, Ann Arbor, MI, 48109

    Michael W. Long

Authors
  1. George G. Pipia
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  2. Michael W. Long
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Pipia, G., Long, M. Human hematopoietic progenitor cell isolation based on galactose-specific cell surface binding. Nat Biotechnol 15, 1007–1011 (1997). https://doi.org/10.1038/nbt1097-1007

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