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Recombinant Proteins of Therapeutic Interest Expressed by Lymphoid Cell Lines Derived from Transgenic Mice

Bio/Technology volume 7, pages 1049–1054 (1989)Cite this article

Abstract

As a new approach to produce human proteins of therapeutic interest we have derived lymphoid cell lines expressing such proteins from transgenic mice, either by generating trans-hybridomas by cell fusion in vitro or by onc gene-mediated immortalisation in vivo of transgenic lymphocytes. The latter procedure could be applied to different cell types for the expression of proteins that require tissue-specific modifications.

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Author information

Author notes

  1. Tim Skern

    Present address: Institut für Biochemie der Universität Wien, Währinger Straße 17, 1090, Vienna, Austria

  2. Andrea Pavirani: Corresponding author.

Authors and Affiliations

  1. Division of Molecular and Cellular Biology, TRANSGENE S.A., 11 rue de Molsheim, 67000, Strasbourg, France

    Andrea Pavirani & Michael Courtney

  2. Laboratoire de Génétique Moléculaire des Eucaryotes, Institut de Chimie Biologique, 11 rue Humann, 67085, Strasbourg Cedex, France

    Marianne Le Meur, Yves Lutz, Richard Lathe, Jean-Paul Fuchs & Pierre Gerlinger

  3. Pulmonary Branch, National Heart, Lung and Blood Institute NIH, Bethesda, MD, 20205, USA

    Ronald G. Crystal

Authors
  1. Andrea Pavirani
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  2. Tim Skern
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  3. Marianne Le Meur
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  4. Yves Lutz
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  5. Richard Lathe
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  6. Ronald G. Crystal
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  7. Jean-Paul Fuchs
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  8. Pierre Gerlinger
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  9. Michael Courtney
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Pavirani, A., Skern, T., Meur, M. et al. Recombinant Proteins of Therapeutic Interest Expressed by Lymphoid Cell Lines Derived from Transgenic Mice. Nat Biotechnol 7, 1049–1054 (1989). https://doi.org/10.1038/nbt1089-1049

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  • DOI: https://doi.org/10.1038/nbt1089-1049

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