Review Article | Published:

New Genetic Methods for Mammalian Cells

Bio/Technologyvolume 6pages11921196 (1988) | Download Citation

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Abstract

The power of genetics for the elucidation of gene function has been clearly established. In mammalian systems, the limited scope of available methods for the genetic manipulation of cultured cells has discouraged the widespread application of genetic approaches for the study of many current issues in molecular biology. Several recent technological developments hold out the promise of facilitating the manipulation of genes in mammalian cells. Three particular areas are emphasized in this review: 1) mutational analysis using nonsense suppression; 2) gene regulation by rapid, controlled amplification; and 3) gene targeting by homologous recombination.

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Author notes

    • John M. Sedivy

    Present address: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510

Affiliations

  1. Center for Cancer Research and the Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts, 02139

    • John M. Sedivy

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https://doi.org/10.1038/nbt1088-1192

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