Cloning of argG from Streptomyces: Loss of Gene in Arg Mutants of S. cattleya


Genetic instability is common in commercial strains of Streptomyces. Streptomyces cattleya segregates Arg mutants at high frequency. The argG gene from wild type S. cattleya was cloned by complementation of argG in E. coli. DNA containing argG was used as a probe in Southern blot analysis of S. cattleya strains. Arg mutants of S. cattleya have deleted the region of DNA encoding the argG gene.

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Meade, H. Cloning of argG from Streptomyces: Loss of Gene in Arg Mutants of S. cattleya. Nat Biotechnol 3, 917–918 (1985).

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