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Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry

Nature Biotechnology volume 15, pages 906–909 (1997)Cite this article

Abstract

Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and α-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.

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  1. Hans von Döhren: (e-mail: hans@cicsg.tk.tu-berlin.de)

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, Technichal University Berlin, Germany

    Marcel Erhard

  2. Max-Planck-Instituteforlnfectionbiology, Germany

    Peter Jungblut

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  1. Marcel Erhard
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  2. Hans von Döhren
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  3. Peter Jungblut
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Erhard, M., von Döhren, H. & Jungblut, P. Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry. Nat Biotechnol 15, 906–909 (1997). https://doi.org/10.1038/nbt0997-906

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