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Recombinant Bacillus thuringiensis Crystal Proteins with New Properties: Possibilities for Resistance Management

Bio/Technology volume 12, pages 915–918 (1994)Cite this article

Abstract

To obtain Bacillus thuringiensis crystal proteins with new properties and to identify the regions involved in insecticidal activity, we generated hybrid genes composed of cryIC and cryIE by in vivo recombination. Analysis of the hybrid proteins showed that domain III of CryIC is involved in the toxicity towards Spodoptera exigua and Mamestra brassicae. Transfer of this domain to CryIE, which is not active against these insects, resulted in a new protein with a broader activity. This hybrid protein binds to different receptors than CryIC, suggesting its use as an alternative for CryIC in resistance management programs.

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References

  1. Höfte, H. and Whiteley, H.R. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242–255.

    PubMed  PubMed Central  Google Scholar 

  2. Li, J., Carroll, J. and Ellar, D.J. 1991. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2. 5 Å resolution. Nature 353: 815–821.

    Article  CAS  PubMed  Google Scholar 

  3. Choma, C.T., Surewicz, W.K., Carey, P.R., Pozsgay, M., Raynor, T. and Kaplan, H. 1990. Unusual proteolysis of the protoxin and toxin from Bacillus thuringiensis. Structural implications. Eur. J. Biochem. 189: 523–527.

    Article  CAS  PubMed  Google Scholar 

  4. Hofmann, C., Vanderbruggen, H., Höfte, H., Van Rie, J., Jansens, S. and Van Mellaert, H. 1988. Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts. Proc. Natl. Acad. Sci. USA. 85: 7844–7848.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Van Rie, J., Jansens, S., Höfte, H., Degheele, D. and Van Mellaert, H. 1990. Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis δ-endotoxins. Appl. Environ. Microbiol. 56: 1378–1385.

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Van Rie, J., McGaughy, W.H., Johnson, D.E., Barnett, D. and Van Mellaert, H. 1990. Mechanism of insect resistance to the microbial insecticide Bacillus thuringiensis. Science 247: 72–74.

    Article  CAS  PubMed  Google Scholar 

  7. Ferré, J., Real, M.D., Van Rie, J., Jansens, S. and Peferoen, M. 1991. Resistance of the Bacillus thuringiensis bioinsecticide in field population of Plutella xylostella is due to a change in a midgut receptor. Proc. Natl. Acad. Sci. USA. 88: 5119–5123.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Visser, B., Van der Salm, T., Van den Brink, W. and Folkers, G. 1988. Genes from Bacillus thuringiensis entomocidus 60.5 coding for insect-specific crystal proteins. Mol. Gen. Genet. 212: 219–224.

    Article  CAS  Google Scholar 

  9. Masson, L., Moar, W. J., Van Frankenhuyzen, K., Bossé, M. and Brousseau, R. 1992. Insecticidal properties of a crystal protein gene product isolated from Bacillus thuringiensis sbsp. kenyae. Appl. Environ. Microbiol. 58: 642–646.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Visser, B., Munsterman, E., Stoker, A. and Dirkse, W.G. 1990. A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific crystal protein. J. Bacteriol. 172: 6783–6788.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Sambrook, J., Fritz, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

  12. Convents, D., Houssier, C., Lasters, I. and Lauwereys, M. 1990. The Bacillus thuringiensis δ-endotoxin. Evidence for a two domain structure of the minimal toxic fragment. J. Biol. Chem. 265: 1369–1375.

    CAS  PubMed  Google Scholar 

  13. Convents, D., Cherlet, M., Van Damme, J., Lasters, I. and Lauwereys, M. 1991. Two structural domains as a general fold of the toxic fragment of the Bacillus thuringiensis δ-endotoxins. Eur. J. Biochem. 195: 631–635.

    Article  CAS  PubMed  Google Scholar 

  14. Ge, A.Z., Shivarova, N.I. and Dean, D. 1989. Location of the Bomhyx mori specificity domain on a Bacillus thuringiensis δ-endotoxin protein. Proc. Natl. Acad. Sci. USA 86: 4037–4041.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Ge, A.Z., Rivers, D., Milne, R.E. and Dean, D. 1991. Functional domains of Bacillus thuringiensis insecticidal proteins. J. Biol. Chem. 266: 17954–17958.

    CAS  PubMed  Google Scholar 

  16. Lee, M.K., Milne, R.E., Ge, A.Z. and Dean, D.H. 1992. Location of a Bombyx-mori receptor binding region on a Bacillus-thuringiensis δ-endotoxin. J. Biol. Chem. 267: 3115–3121.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Dirk Bosch, Bert Schipper, Hilde van der Kleij, Ruud A. de Maagd & Willem J. Stiekema

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  1. Dirk Bosch
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  2. Bert Schipper
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  3. Hilde van der Kleij
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  4. Ruud A. de Maagd
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  5. Willem J. Stiekema
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Correspondence to Dirk Bosch.

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Bosch, D., Schipper, B., Kleij, H. et al. Recombinant Bacillus thuringiensis Crystal Proteins with New Properties: Possibilities for Resistance Management. Nat Biotechnol 12, 915–918 (1994). https://doi.org/10.1038/nbt0994-915

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