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Transgenic Potato Plants Expressing Mammalian 2′–5′ Oligoadenylate Synthetase are Protected From Potato Virus X Infection Under Field Conditions

Bio/Technology volume 11, pages 1048–1052 (1993)Cite this article

Abstract

We cloned and sequenced a rat cDNA encoding the 2′-5′ oligoadenylate synthetase, a component of the mammalian interferon-induced antiviral response, and used Agrobacterium-mediated transformation to generate transgenic potato clones expressing this mammalian enzyme. In transgenic plants infected with potato virus X and followed under field conditions, virus concentrations in leaves and in tubers were significantly lower than in nontransgenic controls. Additionally, virus concentration in the leaves of five transgenic clones and in tubers of one clone was also lower than in transgenic potatoes expressing potato virus X coat protein.

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Author notes

  1. Mart Saarma: Corresponding author.

Authors and Affiliations

  1. Institute of Chemical Physics and Biophysics, Estonian Academy of Sciences, Akadeemia tee 23, EE0026, Tallinn, Estonia

    Erkki Truve, Anu Aaspôllu & Merike Kelve

  2. Kemira OY, Espoo Research Centre, PL 44, FIN-02271, Espoo, Finland

    Jarmo Honkanen, Reetta Puska, Merja Mehto & Pauli Seppänen

  3. Institute of Plant Protection, Agricultural Research Centre, Jokioinen, FIN-31600, Finland

    Anja Hassi

  4. Institute of Biotechnology, University of Helsinki, P.O. Box 45, Karvaamokuja 3A, FIN-00014, Finland

    Teemu H. Teeri & Mart Saarma

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Truve, E., Aaspôllu, A., Honkanen, J. et al. Transgenic Potato Plants Expressing Mammalian 2′–5′ Oligoadenylate Synthetase are Protected From Potato Virus X Infection Under Field Conditions. Nat Biotechnol 11, 1048–1052 (1993). https://doi.org/10.1038/nbt0993-1048

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