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High Level, Stable Production of Recombinant Proteins in Mammalian Cell Culture using the Herpesvirus VP16 Transactivator

Bio/Technology volume 11, pages 1037–1041 (1993)Cite this article

Abstract

We have engineered mammalian cell lines to produce high levels of heterologous proteins by constructing a cell line that expresses the herpesvirus transactivator, VP16. Subsequent stable transfection with a gene of interest under control of a herpesvirus immediate early promoter led to a rapid isolation of cell lines producing between 1 and 20 micrograms of protein/million cells/24 hours. This high level expression is stable for at least five months.

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  1. Paul Hippenmeyer: Corresponding author.

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  1. Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO, 63198

    Paul Hippenmeyer & Maureen Highkin

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Hippenmeyer, P., Highkin, M. High Level, Stable Production of Recombinant Proteins in Mammalian Cell Culture using the Herpesvirus VP16 Transactivator. Nat Biotechnol 11, 1037–1041 (1993). https://doi.org/10.1038/nbt0993-1037

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