Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Review Article
  • Published:

Oligonucleotide Polymorphisms: A New Tool for Genomic Genetics

Bio/Technology volume 6, pages 1061–1064 (1988)Cite this article

Abstract

The development of molecular genetic markers, detecting variation at the DNA level, has opened new horizons in the genetic analyses of both man and agricultural species. Agricultural applications of “Restriction Fragment Length Polymorphisms” (RFLPs), despite their promise, are limited by the formidable costs involved in examining the large populations required. It is the purpose of this contribution to point out that the introduction of oligonucleotide probes into agricultural genetics may provide a solution to the cost problems. Furthermore, the number of detectable “Oligonucleotide Polymorphisms” (OPs) should exceed by an order of magnitude the number amenable to RFLP techniques, thus opening the gates to additional applications.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Beckmann, J.S. and Soller, M. 1986. From Cloned Genes to Genomic Genetics. In: Expert Consultation on Biotechnology for Livestock Production and Health, FAO, Rome (October 1986, in the press).

    Google Scholar 

  2. Botstein, D., White, R., Skolnick, M., and Davis, R.W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314–331.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. In this procedure DNA is digested by restriction enzymes, the fragments generated are separated by size on a gel, transferred to a solid support, and hybridized to a labeled cloned sequence acting as probe (see ref. 15). Point mutations or small structural changes (e. g. deletions, additions, inversions) in the vicinity of the genomic DNA regions homologous to the probe will affect the length of the fragment containing the probe-homologous sequence. This can be detected as a change—termed an RFLP—in the location of the hybridization band on autoradiography.

  4. Beckmann, J.S. and Soller, M. 1986. Restriction fragment length polymorphisms in plant genetic improvement, Oxford Surveys of Plant Molecular and Cell Biology. 3:196–250. B. J. Miflin, (Ed.). Oxford Press, Oxford.

    Google Scholar 

  5. Soller, M. and Beckmann, J.S. 1982. Restriction fragment length polymorphisms and genetic improvement. In: Proceedings of the Second World Congress on Genetics Applied to Livestock Production (Madrid) 6:396–404.

    Google Scholar 

  6. Soller, M. and Beckmann, J.S. 1983. Genetic polymorphism in varietal identification and genetic improvement. Theor. Appl. Genet. 67:25–33.

    Article  CAS  PubMed  Google Scholar 

  7. Burr, B., Evola, S.V., Burr, F.A., and Beckmann, J.S. 1983. The application of restriction fragment length polymorphism to plant breeding, p. 45–59. In: Genetic Engineering: Principles and Methods, Vol. 5. (J. K. Setlow and A. Hollaender, (Eds.). Plenum Press, New York, NY.

    Chapter  Google Scholar 

  8. Hallerman, E., Kashi, Y., Nave, A., Beckmann, J.S., and Soller, M. 1987. Restriction fragment length polymorphisms in dairy cattle and their utilization for genetic improvement. Wld. Rev. Anim. Production 22.

  9. Lebowitz, R.J., Soller, M., and Beckmann, J.S. 1987. Trait-based analyses for the detection of linkage between marker loci and quantitative trait loci in crosses between inbred lines. Theor. Appl. Genet. 72:556–562.

    Article  Google Scholar 

  10. Kashi, Y., Soller, M., Hallermann, E., and Beckmann, J.S. 1986. Restriction fragment length polymorphisms in dairy cattle genetic improvement, p. 57–63. In: Proceedings of the Third World Congress on Genetics Applied to Livestock Production, Vol. 12. Nebraska.

    Google Scholar 

  11. Geldermann, H. 1975. Investigation on inheritance of quantitative characters in animals by gene markers. I. Methods. Theor. Appl. Genet. 46:319–330.

    Article  CAS  PubMed  Google Scholar 

  12. Conner, B.J., Reyes, A.A., Morin, C., Itakura, K., Teplitz, R.L., and Wallace, R.B. 1983. Detection of sickle-cell βs-globin allele by hybridization with synthetic oligonucleotides. Proc. Nat. Acad. Sci. U.S.A. 80:278–282.

    Article  CAS  Google Scholar 

  13. Kazazian, H.H. Jr. 1985. Gene probes: Application to prenatal and postnatal diagnosis of genetic disease. Clin. Chem. 31:1509–1513.

    CAS  PubMed  Google Scholar 

  14. Erlich, H.A., Horn, G.T., Saiki, R.K., Scharf, S.J., Mullis, K.B., and Bugawan, T. 1986. Genetic analysis using enzymatic amplification of specific genomic sequences, p. 102–107, In: DNA Probes, Current Communications in Molecular Biology, L. S. Lerman (Ed.). Cold Spring Harbor Laboratory, NY.

    Google Scholar 

  15. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.

    Article  CAS  PubMed  Google Scholar 

  16. Garbutt, G.J., Wilson, J.T., Schuster, G.S., Leary, J.J., and Ward, D.G. 1985. Clin. Chem. 31:1203–1206.

    CAS  PubMed  Google Scholar 

  17. Jeffreys, A.J. 1979. DNA sequence variants in the G-gamma, A-gamma, delta and beta globin genes of man. Cell 18:1–10.

    Article  CAS  PubMed  Google Scholar 

  18. Murray, J.C., Mills, K.A., Demopulos, C.M., Hornung, S., and Motulsky, A.G. 1984. Linkage disequilibrium and evolutionary relationships of DNA variants (restriction enzyme fragment length polymorphisms) at the serum albumin locus. Proc. Nat. Acad. Sci. U.S.A. 81:3486–3490.

    Article  CAS  Google Scholar 

  19. Myers, R.M., Larin, Z., and Maniatis, T. 1985. Detection of Single Base Substitutions by Ribonuclease Cleavage at Mismatches in RNA: DNA Duplexes. Science 230:1242–1246.

    Article  CAS  PubMed  Google Scholar 

  20. Myers, R.M., Lumelsky, N., Lerman, L.S., and Maniatis, T. 1985. Detection of single base substitutions in total genomic DNA. Nature 313:495–498.

    Article  CAS  PubMed  Google Scholar 

  21. Church, M. and Gilbert, W. 1984. Genomic sequencing. Proc. Nat. Acad. Sci. U.S.A. 81:1991–1995.

    Article  CAS  Google Scholar 

  22. EMBL Nucleotide Sequence Data Library, Release No. 10 (1986).

  23. Wrischnik, L.A., Higuchi, R.G., Stoneking, M., Erlich, H.A., Arnheim, N., and Wilson, A.C. 1987. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA. Nucl. Acid Res. 15:529–541.

    Article  CAS  Google Scholar 

  24. Bar-Joseph, M., Segev, D., Blickle, W., Yesodi, V., Franck, A., and Rosner, A. 1986. Application of synthetic DNA probes for the detection of viroids and viruses, p. 13–23. In: Developments in Applied Biology. Jones, R. A. C. and Torrance, L. (Eds.). Assoc. of Applied Biologists, Warwick, U. K.

    Google Scholar 

  25. Stam, P. and Zeven, A.C. 1981. The theoretical proportion of the donor genome in near-isogenic lines of self-fertilizers bred by back-crossing. Euphytica 30:227–238.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, 50250, Israel

    Jacques S. Beckmann

Authors
  1. Jacques S. Beckmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beckmann, J. Oligonucleotide Polymorphisms: A New Tool for Genomic Genetics. Nat Biotechnol 6, 1061–1064 (1988). https://doi.org/10.1038/nbt0988-1061

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0988-1061

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing