Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Searching Sequence Space

Bio/Technology volume 13, pages 722–723 (1995)Cite this article

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Stemmer, W.P.C. 1995. Searching sequence space. Bio/Technology 13: 549–553.

    CAS  Google Scholar 

  2. Stemmer, W.P.C. 1994. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA 91: 10747–10751.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Stemmer, W.P.C. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389–391.

    Article  CAS  PubMed  Google Scholar 

  4. Arkin, A.P. and Youvan, D.C. 1992. An algorithm for protein engineering: Simulation of recursive ensemble mutagenesis. Proc. Natl. Acad. Sci USA 89: 7811–7815.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Youvan, D.C., Arkin, A.P., and Yang, N.M. 1992. Recursive ensemble mutagenesis: A combinatorial optimization technique for protein engineering. In Parallel Problem Solving from Nature. B. Manderick. ed. Elsevier Publishing Co., New York, pp.401–410.

    Google Scholar 

  6. Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R., and Sauer, R.T. 1991. Random mutagenesis of protein sequences using oligonucleotide cassettes. Meth. Enzymol. 208: 564–587.

    Article  CAS  Google Scholar 

  7. Oliphant, A.R., Nussbaum, A.L., and Struhl, K. 1986. Cloning of random sequence oligonucleotides. Gene 44: 177–183.

    Article  CAS  PubMed  Google Scholar 

  8. Fuellen, G. and Youvan, D.C. 1994. Genetic algorithms and recursive ensemble mutagenesis in protein engineering. Complexity International, http://life.anu.edu.au/ci/voll/fuellen/REM.html.

  9. Goldman, E.R. and Youvan, D.C. 1992. An algorithmically optimized combinatorial library screened by digital imaging spectroscopy. Bio/Technology 10: 1557–1561.

    CAS  Google Scholar 

  10. Youvan, D.C. 1994. Imaging sequence space. Nature 369: 79–80.

    Article  CAS  PubMed  Google Scholar 

  11. Delagrave, S., Goldman, E.R., and Youvan, D.C. 1993. Recursive ensemble mutagenesis. Protein Engineering 6: 327–331.

    Article  CAS  PubMed  Google Scholar 

  12. Youvan, D.C., Goldman, E., Delagrave, S., and Yang, M.M. 1994. Digital imaging spectroscopy for massively parallel screening of mutants. Meth. Enzymol. 246: 732–748.

    Article  Google Scholar 

  13. Delagrave, S. and Youvan, D.C. 1993. Searching sequence space: Exponential ensemble mutagenesis. Bio/Technology 11: 1548–1552.

    CAS  Google Scholar 

  14. Goldman, E., Fullen, G., and Youvan, D.C. 1994. Estimation of protein function from combinatorial mutagenesis by decision algorithms and neural networks. Drug Dev. Res. 33: 125–132.

    Article  CAS  Google Scholar 

  15. Delagrave, S.E., and Youvan, D.C. 1995. Context dependence of phenotype prediction and diversity in combinatorial mutagenesis. Protein Engineering. 8: 237–241.

    Article  CAS  PubMed  Google Scholar 

  16. Yang, M.M., Coleman, W.J. and Youvan, D.C. 1990. Genetic coding algorithms for membrane proteins. In Structure and Function of the Bacterial Photosynthetic Reaction Center. Springer Series in Chemical Physics, M.E. Michel-Beyerle, ed. pp. 209–217.

    Chapter  Google Scholar 

  17. Delagrave, S., Hawtin, R.E., Silva, C.M., Yang, M.M., and Youvan, D.C. 1995. Red-shifted excitation mutants of the green fluorescent protein. Bio/Technology 13: 151–154.

    CAS  Google Scholar 

  18. Balint, R.F. and Larrick, J.W. 1993. Antibody engineering by parsimonious mutagenesis. Gene 137: 109–118.

    Article  CAS  PubMed  Google Scholar 

  19. Chen, K. and Arnold, F.H. 1993. Tuning the activity of an enzyme for unusual environments: Sequential random mutagenesis of subtilisin E for catalysis in dimethyl-formamide. Proc. Natl. Acad. Sci. USA 90: 5618–56.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Palo Alto Institute of Molecular Medicine, 2462 Wyandotte Street, Mountain View, CA, 94043

    Douglas C. Youvan

Authors
  1. Douglas C. Youvan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Youvan, D. Searching Sequence Space. Nat Biotechnol 13, 722–723 (1995). https://doi.org/10.1038/nbt0895-722

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0895-722

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing