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A New Tobacco Mosaic Virus Vector and its Use for the Systemic Production of Angiotensin-I-Converting Enzyme Inhibitor in Transgenic Tobacco and Tomato

Bio/Technology volume 11, pages 930–932 (1993)Cite this article

Abstract

We have developed a new tobacco mosaic virus (TMV) RNA vector and have used it initially to systemically produce an angiotensin-I-converting enzyme inhibitor peptide (ACEI) in tobacco and tomato plants. This vector incorporates a six base 3′ context sequence, which permits readthrough of the stop codon for the TMV 130K protein gene, inserted between the stop codon for the coat protein (CP) gene and ACEI gene. In contrast to previous TMV RNA vectors, the new vector produced both an intact CP and a fused protein consisting of CP and ACEI (CP-ACEI). As a result, the vector could form virus particles and spread systemically from inoculated to non-inoculated leaves. In tomato plants, production of ACEI in fruit was also achieved.

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References

  1. Donson, J., Kearney, C.M., Hilf, M.E. and Dawson, W.O. 1991. Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector. Proc. Natl. Acad. Sci. USA 88: 7204–7208.

    Article  CAS  Google Scholar 

  2. Takamatsu, N., Ishikawa, M., Meshi, T. and Okada, Y. 1987. Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA. EMBO J. 6: 307–311.

    Article  CAS  Google Scholar 

  3. Takamatsu, N., Watanabe, Y., Yanagi, H., Meshi, T. 1990. Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector. FEBS Lett. 269: 73–76.

    Article  CAS  Google Scholar 

  4. Dawson, W.O., Lewandowski, D.J., Hilf, M.E., Bubrick, P. et al. 1989. A tobacco mosaic virus-hybrid expresses and loses an added gene. Virology 172: 285–292.

    Article  CAS  Google Scholar 

  5. Saito, T., Yamanaka, K. and Okada, Y. 1990. Long-distance movement of tobacco mosaic virus mutants. Virology 176: 329–336.

    Article  CAS  Google Scholar 

  6. Kumagai, M.H., Turpen, T.H., Weinzettl, N., Della-Cioppa, G. et al. 1993. Rapid, high-level expression of biologically active α-trichosanthin in transfected plants by an RNA viral vector. Proc. Natl. Acad. Sci. USA 90: 427–430.

    Article  CAS  Google Scholar 

  7. Skuzeski, J.M., Nichols, L.M., Gesteland, R.F. and Atkins, F. 1991. The signal for a leaky UAG codon in several plant viruses includes the two downstream codons. J. Mol. Biol. 218: 365–373.

    Article  CAS  Google Scholar 

  8. Karaki, H., Doi, K., Sugano, S., Uchiwa, H. et al. 1990. Antihypertensive effect of tryptic hydrolysate of milk casein in spontaneously hypertensive rats. Comp. Biochem. Physiol. 96C: 367–371.

    CAS  Google Scholar 

  9. Meshi, T., Ishikawa, M., Motoyoshi, F., Semba, K. and Okada, Y. 1986. In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus. Proc. Natl. Acad. Sci. USA 83: 5043–5047.

    Article  CAS  Google Scholar 

  10. Meshi, T., Watanabe, Y. and Okada, Y. 1992. Molecular pathology of tobacco mosaic virus revealed by biologically active cDNAs, p.149–186. In: Genetic Engineering with Plant Viruses. Wilson, T. M. A. and Davis, J. W. (Eds.). CRC Press, Boca Raton, FL.

    Google Scholar 

  11. Watanabe, Y., Meshi, T. and Okada, Y. 1987. Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method. FEBS Lett. 219: 65–69.

    Article  CAS  Google Scholar 

  12. Mattehews, R.E.F. 1981. Plant Virology. Academic Press,NY.

  13. Goelet, P., Lomonossoff, G.P., Butler, P.J.G., Akam, M.E. 1982. Nucleotide sequence of tobacco mosaic virus RNA. Proc. Nad. Acad. Sci. USA 79: 5818–5822.

    Article  CAS  Google Scholar 

  14. Bloomer, A.C., Champness, J.N., Bricogne, G., Staden, R. and Klug, A. 1978. Protein disk of tobacco mosaic virus at 2.8 A resolution showing the interactions within and between subunits. Nature 276: 362–368.

    Article  CAS  Google Scholar 

  15. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning; A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, NY.

    Google Scholar 

  16. Verver, J., Goldbach, R., Garcia, J.A. and Vos, P. 1987. In vitro expression of a full-length DNA copy, of cowpea mosaic virus B RNA: Identification of the BRNA-encoded 24kDa protein as a viral protease. EMBO J. 6: 549–554.

    Article  CAS  Google Scholar 

  17. Otsuki, Y., Takebe, I., Ohno, T., Fukuda, M. and Okada, Y. 1977. Reconstitution of tobacco mosaic virus rods occurs bidirectionally from an internal initiation region: Demonstration by electron microscopic serology. Proc. Nad. Acad. Sci. USA 74: 1913–1917.

    Article  CAS  Google Scholar 

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  1. Hiroshi Hamamoto: Corresponding author.

Authors and Affiliations

  1. Biochemistry Laboratory, Kanebo Ltd., Kotobukicho, Odawara, Kanagawa, 250, Japan

    Hiroshi Hamamoto, Yoshinori Sugiyama, Noriaki Nakagawa, Eiji Hashida, Yuji Matsunaga & Shizume Takemoto

  2. Department of Biosciences, Teikyo University, Toyosato-dai, Utsunomiya, Tochigi, 320, Japan

    Yuichiro Watanabe & Yoshimi Okada

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  1. Hiroshi Hamamoto
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  2. Yoshinori Sugiyama
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Hamamoto, H., Sugiyama, Y., Nakagawa, N. et al. A New Tobacco Mosaic Virus Vector and its Use for the Systemic Production of Angiotensin-I-Converting Enzyme Inhibitor in Transgenic Tobacco and Tomato. Nat Biotechnol 11, 930–932 (1993). https://doi.org/10.1038/nbt0893-930

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