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Hyperproduction of Tryptophan in Corynebacterium glutamicum by Pathway Engineering

Bio/Technology volume 11, pages 921–925 (1993)Cite this article

Abstract

We have performed pathway engineering of a tryptophan-producing strain of Corynebacterium glutamicum using cloned homologous genes. Plasmid-mediated amplification of a feedback-insensitive 3-deoxy-D-arabino-heptulospnate 7-phosphate synthase (DS) in the strain augmented the carbon flow down the common aromatic pathway, but caused concurrent excretion of chorismate, the last metabolite. Alternatively, introduction of a plasmid coexpressing the set of tryptophan-biosynthetic enzymes along with DS resulted hi formation of anthranilate, the first intermediate hi the tryptophan branch, as another byproduct. However, mutatkmal alterations of plasmid-encoded anthranilate synthase and anthranilate phosphoribosyltransferase, which rendered them insensitive to tryptophan inhibition, led to efficient channelling of carbon through the overall pathway to tryptophan. This engineered strain displays a 54 percent yield increase relative to its parent and produces 43 grams of tryptophan per liter.

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  1. Ryoichi Katsumata: Corresponding author.

Authors and Affiliations

  1. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Asahi-machi, Machida-shi, Tokyo, 194, Japan

    Ryoichi Katsumata & Masato Ikeda

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  1. Ryoichi Katsumata
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  2. Masato Ikeda
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Katsumata, R., Ikeda, M. Hyperproduction of Tryptophan in Corynebacterium glutamicum by Pathway Engineering. Nat Biotechnol 11, 921–925 (1993). https://doi.org/10.1038/nbt0893-921

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