Research Paper | Published:

Field Resistance of Transgenic Russeet Burbank Potato to Effects of Infection by Potato Virus X and Potato Virus Y

Bio/Technology volume 8, pages 750754 (1990) | Download Citation

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Abstract

Transgenic Russet Burbank potato plants expressing the coat protein genes of potato virus X (PVX) and potato virus Y (PVY) were transplanted into the field after propagation in tissue culture. Virus resistance, plant growth and tuber yield were determined. Our results show that expression of PVX and PVY CP genes confer a very high level of resistance to PVX and PVY infection in clone 303, one of the four different transgenic potato clones tested. After inoculation with PVX and PVY, tuber yield of control Russet Burbank plants decreased, while the yield of clone 303 was unaffected. Four different uninoculated transgenic clones had tuber yields as high as control Russet Burbank. These results confirm that resistance to PVX and PVY is effective in the field and can prevent yield losses due to dual infection by these viruses.

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References

  1. 1.

    and 1987. Survey of Properties and Symptoms. In: Viruses of Potatoes and Seed potato Production, p. 84–113. J. A. deBokx and J. P. H. Van der Want, (Eds). Wageningen, Netherlands.

  2. 2.

    1986. Potato Virus Y. In: Compendium of Potato Diseases, p. 70–71 . W. J. Hooker, (Ed.) American Phytopathological Society.

  3. 3.

    1986. Potato Virus X. In: Compendium of Potato Diseases, p. 72–74. W. J. Hooker, (Ed). American Phytopathological Society.

  4. 4.

    , , , , , and 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.

  5. 5.

    , , , , , and 1987. Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO J. 6: 1181–1188.

  6. 6.

    , , , , , , , and 1987. Expression of alfalfa mosaic virus RNA4 in transgenic plants confers virus resistance. EMBO J. 6: 1845–1851.

  7. 7.

    , and 1987. Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159: 299–305.

  8. 8.

    , , , , and 1988. Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6: 549–557.

  9. 9.

    , , , and 1988. Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO J. 7: 1273–1280.

  10. 10.

    , , and 1988. Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross protect against alfalfa mosaic virus infection. Virology 164: 383–389.

  11. 11.

    and 1988. Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167: 649–652.

  12. 12.

    , , and 1989. Genetic Engineering of plants for Viral Disease Resistance. In: Cell Culture and Somatic Cell Genetics, p. 405–423. J. Schell and I. Vasil (Eds.) Academic Press, Inc., San Diego, California.

  13. 13.

    , , , , , and 1990. Engineering resistance to Multiple virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Y in transgenic Russet Burbank potato. Bio/Technology 8: 127–134.

  14. 14.

    , , , , , , , , , , and 1988. Virus tolerance, plant growth and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.

  15. 15.

    , , , and 1989. The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technoiogy 7: 273–278.

  16. 16.

    , and 1983. Results of experiments on the purification of potato virus Y. Phytopathology 73: 794–797.

  17. 17.

    and 1972. Relative antigenic specificities of two PVX strains and their d-protein oligomers. Virology 47: 54–60.

  18. 18.

    and 1989. Protection against potyvirus infection in transgenic plants: Evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262.

  19. 19.

    and 1975. A study of the powers of several methods of multiple comparisons. J. of the Amer. Statistical Association 70: 351.

  20. 20.

    , and 1955. Comparison of local-lesion and electron microscope particle count methods for assay of potato virus X from plants doubly infected by potato viruses X and Y. Virology 1: 28–39.

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Author information

Author notes

    • Nilgun E. Tumer

    Corresponding author.

Affiliations

  1. Plant Sciences Department, Monsanto comapny, 700 Chesterfield Village Parkway, St. Louis, MO 63188.

    • Wojciech Kaniewski
    • , Cliff Lawson
    • , Bernard Sammons
    • , Lisa Haley
    • , Jesse Hart
    • , Xavier Delannay
    •  & Nilgun E. Tumer

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DOI

https://doi.org/10.1038/nbt0890-750

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