Abstract
We have devised a direct selection for auxotrophic mutant isolation, and report here its use to obtain a xanthine-requiring mutant of B. subtilis that shows significantly improved inosine production. Insertion of a chloramphenicol acetyl transferase gene into a cloned B. subtilis IMP dehydrogenase gene on pBR322 was followed by transformation of inosine-guanosine producing B. subtilis with a fragment of the mutagenized plasmid. All chloramphenicol resistant transformants were xanthine dependent and accumulated almost twice as much inosine as the parental strain.
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Miyagawa, Ki., Kanzaki, N., Kimura, H. et al. Increased Inosine Production by a Bacillus subtilis Xanthine-Requiring Mutant Derived by Insertional Inactivation of the IMP Dehydrogenase Gene. Nat Biotechnol 7, 821–824 (1989). https://doi.org/10.1038/nbt0889-821
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DOI: https://doi.org/10.1038/nbt0889-821
