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In vitro hydrogen production by glucose dehydrogenase and hydrogenase

Nature Biotechnology volume 14, pages 872–874 (1996)Cite this article

Abstract

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity.

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References

  1. Lepkowski, W. 1995. The Politics of Hydrogen. Chem. & Eng. News 73: 26–49.

    Article  Google Scholar 

  2. Lawler, A. 1995. Walker Bill to boost hydrogen sparks democratic grumbling. Science 267: 613.

    Article  CAS  PubMed  Google Scholar 

  3. Lee, J.W. and Greenbaum, E. 1995. Bioelectronics and biometallocatalysis for production of fuels and chemicals by photosynthetic water splitting. Appl. Biochem. Biotechnol. 51/52: 295–305.

    Article  CAS  Google Scholar 

  4. Kitani, O. and Hall, C.W. 1989. Biomass Handbook. Gordon and Breach Science Publishers, New York.

    Google Scholar 

  5. Taguchi, F., Mizukami, N., Yamada, K., Hasegawa, K., and Saito-Taki, T. 1995. Direct conversion of cellulosic materials to hydrogen by Clostridium sp. strain no. 2. Enzyme Microb. Technol. 17: 147–150.

    Article  CAS  Google Scholar 

  6. Harrison, D.C. 1931. Glucose dehydrogenase: A new oxidising enzyme from animal tissues. Biochem. J. 26: 1016–1027.

    Article  Google Scholar 

  7. Tranulis, M.A., Christopherson, B., and Borrebaek, B. 1994. Glucose dehydrogenase in beef (Bos taurus) and rainbow trout (Oncorhynchus mykiss) liver: a comparative study. Comp. Biochem. Physiol. 109B: 427–435.

    CAS  Google Scholar 

  8. Pauly, H.E. and Pfleiderer, G. 1977. Conformational and functional aspects of the reversible dissociation and denatuation of glucose dehydrogenase. Biochemistry 16: 4599–4604.

    Article  CAS  PubMed  Google Scholar 

  9. Smith, L.D., Budgen, N., Bungard, S.J., Danson, M.J., and Hough, D.W. 1989. Purification and characterization of glucose dehydrogenase from the thermoacidophilic Archaebacterium Thermoplasma acidophilum . Biochem. J. 261: 973–977.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Greenbaum, E. 1980. Simultaneous photoproduction of hydrogen and oxygen by photosynthesis. Biotechnol. Bioeng. Symp. 10: 1–14.

    CAS  Google Scholar 

  11. Egerer, P., Günther, H., and Simon, H. 1982. On the hydrogen-deuterium exchange reaction catalyzed by the soluble hydrogenase from Alcaligenes eutrophus H16 in the free and immobilized state. Biochim. Biophys. Acta 703: 149–157.

    Article  CAS  PubMed  Google Scholar 

  12. Ma, K., Zhou, Z.H., and Adams, M.W.W. 1994. Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH. FEMS Microbiol. Lett. 122: 245–250.

    Article  CAS  Google Scholar 

  13. Bright, J.R., Byrom, D., Danson, M.J., Hough, D.W., and Towner, P. 1993. Cloning, sequencing and expression of the gene encoding glucose dehydrogenase from the thermophilic Archaeon Thermoplasma acidophilum . Eur. J. Biochem. 211: 549–554.

    Article  CAS  PubMed  Google Scholar 

  14. Bryant, F.O. and Adams, M.W.W. 1989. Characterization of hydrogenase from the hyperthermophilic Archaebacterium, Pyrococcus furiosus . J. Biol. Chem. 264: 5070–5079.

    CAS  PubMed  Google Scholar 

  15. Adams, M.W.W., unpublished material.

  16. Pedroni, P., Volpe, A.D., Galli, G., Mura, G.M., Pratesi, C., and Grandi, G. 1995. Characterization of the locus wncoding the [Ni-Fe] aulfhydrogenase from the Archaeon Pyrococcus furiosus: evidence for a relationship to bacterial sulfite reductases. Microbiol. 141: 449–458.

    Article  CAS  Google Scholar 

  17. John, J., Crennell, S.J., Hough, D.W., Danson, M.J., and Taylor, G.L. 1994. The crystal structure of glucose dehydrogenase from Thermoplasma acidophilum . Structure 2: 385–393.

    Article  CAS  PubMed  Google Scholar 

  18. Danson, M.J. 1993. Central metabolism of the Archaea, pp. 1–24 in The biochemistry of Archaea (Archaebacteria). Kates, M., Kushner, D.J., and Matheson, A.T. (eds.). Elsevier, Amsterdam, the Netherlands.

    Google Scholar 

  19. Walker L.P. and Wilson, D.B. (eds.). 1991. Enzymatic hydrolysis of cellulose. Elsevier Applied Science, London and New York.

    Google Scholar 

  20. Sodium gluconate handbook. 1994. PMP Fermentation Products, Inc., Chicago, IL

  21. Woodward, J., Stephan, L.M., Koran, L.J., Jr, Wong, K.K.Y., and Saddler, J.N. 1994. Enzymatic separation of high-quality uninked pulp fibers from recycled newspaper. Bio/Technology 12: 905–908.

    CAS  Google Scholar 

  22. Data provided by the Oak Ridge Utility District, Oak Ridge, TN.

  23. Greenbaum, E. 1984. Biophotolysis of water: the light saturation curves. Photobiochem. Photobiophys. 8: 323–332

    CAS  Google Scholar 

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Author notes

  1. Jonathan Woodward: email: oop@ornl.gov

Authors and Affiliations

  1. Chemical Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831-6194

    Jonathan Woodward & Susan M. Mattingly

  2. School of Biology and Department of Biochemistry, University of Bath, Bath, BA2 7AY, UK

    Michael Danson, David Hough & Nicholas Ward

  3. Department of Biochemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA, 30602

    Michael Adams

Authors
  1. Jonathan Woodward
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  2. Susan M. Mattingly
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  3. Michael Danson
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  4. David Hough
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  5. Nicholas Ward
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  6. Michael Adams
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Woodward, J., Mattingly, S., Danson, M. et al. In vitro hydrogen production by glucose dehydrogenase and hydrogenase. Nat Biotechnol 14, 872–874 (1996). https://doi.org/10.1038/nbt0796-872

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