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High Level Expression of Tissue Inhibitor of Metalloproteinases in Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification

Bio/Technology volume 8, pages 662–667 (1990)Cite this article

Abstract

We have used a glutamine synthetase (GS) gene as an amplifiable marker in Chinese hamster ovary (CHO) cells. GS was combined with an efficient transcription unit to produce tissue inhibitor of metalloproteinases (TIMP). Initial transfectant cell-lines selected using a GS gene secreted up to 9μg TIMP/106 cells/24h. After one round of GS gene amplification expression levels of 110μg TIMP/106 cells/24h were achieved. These GS gene amplified CHO cells, when adapted to grow in suspension, accumulated 180mg/l in shake flask culture. This system therefore provides a rapid method of achieving high level gene expression in mammalian cells.

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Authors and Affiliations

  1. Celltech Limited, 216 Bath Road Slough, SL1 4EN, Berkshire, U.K.

    M. I. Cockett, C. R. Bebbington & G. T. Yarranton

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  1. M. I. Cockett
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  2. C. R. Bebbington
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  3. G. T. Yarranton
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Cockett, M., Bebbington, C. & Yarranton, G. High Level Expression of Tissue Inhibitor of Metalloproteinases in Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification. Nat Biotechnol 8, 662–667 (1990). https://doi.org/10.1038/nbt0790-662

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