Research Paper | Published:

Use of a Cell Recycle Reactor to Increase Production of a Proteolysis-Susceptible Peptide Secreted from Recombinant Saccharomyces cerevisiae

Bio/Technology volume 8, pages 639643 (1990) | Download Citation

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Abstract

Operation of a continuous microbial fermentor with cell recycle can significantly reduce degradation-associated loss of a secreted protein product. Under continuous fermentation conditions, proteolysis of a recombinant growth hormone releasing factor (GRF) analog secreted by S. cerevisiae was first order with respect to GRF concentration. The maximal GRF concentration was increased from 5 mg/l to 30 mg/l by the use of a cell recycle reactor, and volumetric productivity was increased more than 10-fold to an average of 10 mg/l−1/h−1. A mathematical model shows that increased productivity in the cell recycle reactor results from a reduced degradation rate and a shorter residence time of the product in the fermentor.

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Affiliations

  1. The Salk Institute Biotechnology/Industrial Associates, Inc. (SIBIA), P.O. Box 85200, San Diego, CA 92138.

    • R.S. Siegel
    •  & R.A. Brierley

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DOI

https://doi.org/10.1038/nbt0790-639

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