Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Paper
  • Published:

Immunomodulatory Components Present in IMREG-1, an Experimental Immunosupportive Biologic

Bio/Technology volume 6, pages 810–815 (1988)Cite this article

An Erratum to this article was published on 01 September 1988

Abstract

Human leukocyte dialysates contain substances capable of affecting human immune function. A low molecular weight subfraction derived from human leukocyte dialysates, IMREG-1, has been shown to enhance cell mediated immunity, and is under clinical evaluation as an immunosupportive agent in AIDS/ARC patients. We report here the isolation and amino acid sequences of two immunologically active peptides, Tyr-Gly and Tyr-Gly-Gly present in IMREG-1. These peptides have not previously been demonstrated to affect the immune system, and it is of interest to note that they are identical to the amino terminal ends of the enkephalins.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Gottlieb, A.A. and Sutcliffe, S.B. 1982. In vivo modification of delayed type hypersensitivity by small molecular weight components derived from human leukocytes: partial purification of components causing amplification of response. Clin. Exp. Immunol. 50:434–441.

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Gottlieb, A.A., Farmer, J.L., Matzura, C.T., Hester, R.B., and Rosenberg, J.S. 1984. Modulation of human T cell production of migration inhibitory lymphokines by cytokines derived from human leukocyte dialysates. J. Immunol. 132:256–260.

    CAS  PubMed  Google Scholar 

  3. Gottlieb, A.A. 1986. Immunoamplifiers and processes for the extraction thereof. U. S. Patent 4,616,079.

  4. Gottlieb, A.A., Gottlieb, M.S., and Scholes, V.E. 1987. Reconstitution of immune function in AIDS/ARC, p. 261–274. In: Concepts in Immunopathology. J. M. Cruse and R. E. Lewis, Jr. (Eds.) S. Karger, Basel.

    Google Scholar 

  5. Gottlieb, A.A., Farmer, J.L., and Nishihara, T. 1986. Endogenous immunomodulators from human leukocytes as agents for immunorestoration in patients with AIDS, p. 287–299. In: Viruses, Immunity and Immunodeficiency. A. Szentivanyi and H. Friedman (Eds) Plenum Press, New York.

    Chapter  Google Scholar 

  6. Gottlieb, A.A., Sinha, S.K., Levine, A., Gottlieb, M.S., Gill, P., and Kern, C.H. Clinical observations on IMREG-1, an immunosupportive biologic. 1988. Submitted.

  7. Hunkapiller, M.W. and Hood, L.E. 1978. Direct microsequence analysis of polypeptides using an improved sequentor, a nonprotein carrier (Polybrene), and high pressure liquid chromatography. Biochemistry 17:2124–2133.

    Article  CAS  Google Scholar 

  8. Hunt, D.F., Yates, J.R., III, Shabanowitz, J., Winston, S., and Hauer, C.R. 1986. Protein sequencing by tandem mass spectrometry. Proc. Natl. Acad. Sci. USA 83:6233–6237.

    Article  CAS  Google Scholar 

  9. Heijnen, C.J., Bevers, C., Kavelaars, A., and Ballieux, R.E. 1986. Effect of α-endorphin on the antigen-induced primary antibody response of human blood B cells in vitro. J. Immunol. 136:213–216.

    CAS  PubMed  Google Scholar 

  10. McKelvy, J.F. 1986. Inactivation and metabolism of neuropeptides. Ann. Rev. Neurosci. 9:415–34.

    Article  CAS  Google Scholar 

  11. Fauchere, J-L. 1986. Elements for the rational design of peptide drugs. Adv. in Drug Res. 15:29–69.

    CAS  Google Scholar 

  12. Burbach, J.P.H. 1984. Action of proteolytic enzymes on lipotropins and endorphins: biosynthesis, biotransformation and fate. Pharmacol. Ther. 24:321–354.

    Article  CAS  Google Scholar 

  13. Smith, E.M. and Blalock, J.E. 1981. Human lymphocyte production of corticotropin and endorphin-like substances: association with leukocyte interferon. Proc. Natl. Acad. Sci. USA 78:7530–7534.

    Article  CAS  Google Scholar 

  14. Smith, E.M., Harbour-McMenamin, D., and Blalock, J.E. 1985. Lymphocyte production of endorphins and endorphin-mediated immunoregulatory activity. J. Immunol. 135:779–782.

    Google Scholar 

  15. Plotnikoff, N.P., Faith, R.E., Murgo, A.J., and Good, R.A. 1986. Enkephalins and Endorphins: Stress and the Immune System. Plenum Press, New York, NY.

    Book  Google Scholar 

  16. Wybran, J., Schandene, L., Van Vooren, J-P., Vandermoten, G., Latinne, D., Sonnet, J., DeBruyere, M., Taelman, H., and Plotnikoff, N.P. 1987. Ann. NY Acad. Sci. 496:108–114.

    Article  CAS  Google Scholar 

  17. Ascher, M.S., Gottlieb, A.A., and Kirkpatrick, C.H. (Eds) 1976. Transfer Factor: Basic Properties and Clinical Applications. Academic Press, New York, NY.

    Google Scholar 

  18. Sinha, S.K., Gottlieb, A.A., Hochadel, J.F., Dischinger, H.C. and Akerstrom, V. 1988. Quantitation of picogram amounts of tyrosine-containing small peptides present in physiological samples. The FASEB J. 2:A1543.

    Google Scholar 

  19. Chang, T.W., McKinney, S., Liu, V., Kung, P.C., Vilcek, J. and Le, J. 1984. Use of monoclonal antibodies as sensitive and specific probes for biologically active human γ-interferon. Proc. Natl. Acad. Sci. USA 81:5219–5222.

    Article  CAS  Google Scholar 

  20. Vilcek, J., Henriksen-Destefano, D., Siegel, D., Klion, A., Robb, R.J., and Le, J. 1985. Regulation of IFN-γ induction in human peripheral blood cells by exogenous and endogenously produced interleukin-2. Journal of Immunology. 135:1851–1856.

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. A. Arthur Gottlieb: Corresponding author.

Authors and Affiliations

  1. Imreg, Inc., 144 Elk Place, Suite 1400, New Orleans, Louisiana, 70112

    Sudhir K. Sinha, Robert C. Sizemore & A. Arthur Gottlieb

Authors
  1. Sudhir K. Sinha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert C. Sizemore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Arthur Gottlieb
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sinha, S., Sizemore, R. & Gottlieb, A. Immunomodulatory Components Present in IMREG-1, an Experimental Immunosupportive Biologic. Nat Biotechnol 6, 810–815 (1988). https://doi.org/10.1038/nbt0788-810

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0788-810

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing