Abstract
An efficient transformation system was developed for tomato by studying several factors that affect the rate of Agrobacterium mediated transformation of explant tissue. Cotyledons of tomato were co–cultivated with Agrobacterium tumefaciens harboring a binary vector with two neomycin phosphotransferase (NPT) II genes and a mutant aroA gene. Over 100 transgenic plants were regenerated and rooted on medium containing kanamycin and eighty percent of these plants exhibited both NPT II enzyme activity and produced the mutant aroA protein. Progeny of aroA positive plants were tolerant to glyphosate at concentrations of 0.84 kg active ingredient/ha. The tolerance phenotype segregated in a manner consistent with Mendelian inheritance.
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References
Baird, D.D., Upshiurch, R.P., Homesely, W.B. and Franz, J.E. 1971. Introduction of a new broad spectrum post-emergence herbicide class with utility for herbaceous perennial weed control. Proc. North Cent. Weed Control Conf. 26: 6468.
Barker, R.F., Idler, K.B., Thompson, D.V. and Kemp, J.D. 1983. Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol. Biol. 2: 335–350.
Bernatzky, R. and Tanksley, S.D. 1986. Towards a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112: 887–898.
Bevan, M., Flavell, R.B., and Chilton, M.-D. 1983. A chimeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184–187.
Comai, L., Sen, L., and Stalker, D. 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221: 370–371.
Comai, L., Facciotti, D., Hiatt, W.R., Thompson, G., Rose, R., and Stalker, D. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744.
Dellaporta, S.L., Wood, J., and Hicks, J.B. 1983. A plant DNA minipreparation: version II. Plant Mol. Bio. Rep. 1: 19–21.
Ditta, G., Stanfield, S., Corbin, D., Helinski, D. 1980. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci USA 77: 7347–7351.
Fillatti, J.J., Sellmer, J., McCown, B., Haissig, B., and Comai, L. 1987. Agrobacterium mediated transformation and regeneration of Populus. Mol. Gen. Genet. 206: 192–199.
Folmar, L.C., Sanders, H.O., Julin, A.M. 1979. Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates. Arch. Environ. Contam. Toxicol. 8: 269–278.
Fraley, R.T., Rogers, S.G., Horsch, R.B., Sanders, P., Flick, J., Adams, S., Bittner, M., Brand, L., Fink, C., Fry, J., Galluppi, G., Goldberg, S., Hoffmann, N., and Woo, S. 1983. Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80: 4803–4807.
Garfinkel. 1980. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J. Bacteriol. 144: 732–743.
Herrera-Estrella, L., Depicker, A., Van Montagu, M. and Schell, J. 1983. Expression of chimeric genes transferred into plant cells using Ti plasmid-derived vector. Nature 303: 209–213.
Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. 1983. A binary plant vector strategy based on separation of vir and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180.
Horsch, R.B., Fry, J.B., Hoffmann, N.L., Wallroth, M., Eichholtz, D., Rogers, S.G. and Fraley, R.T. 1985. A simple and general method for transferring genes into plants. Science 227: 1229–1231.
Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, K., Fraley, R. 1986. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Reports 5: 81–84.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. and Green, M.R. 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acid. Res. 12: 7035–7056.
Reiss, B., Sprengle, R., Will, H. and Schaller, H. 1984. A new sensitive method for qualitative and quantitative assay of neomycin phospho-transferase in crude cell extracts. Gene 30: 211–217.
Rick, C.M. 1983. Tomato (Lycopersicon) In: Isozymes in Plant Genetics and Breeding. S. D. Tanksley and T.J. Orton (eds.). Elsevier Co., Amsterdam.
Shah, D., Horsch, R., Klee, H., Kishore, G., Winter, J., Tumer, N., Hironaka, C., Sanders, P., Gasser, C., Aykent, S., Siegel, N., Rogers, S. and Fraley, R. 1982. Engineering herbicide tolerance in transgenic plants. Sci. 233: 478–481.
Shahin, E.A., Spielmann, A., Sukhapinda, K., Simpson, R.B. and Yashar, M. 1986. Transformation of cultivated alfalfa using disarmed Agrobacterium tumefaciens. Crop Sci. 26: 1235–1239.
Sinich, 1982. Hypothesis testing, p. 313–362. In: Statistics by Example. Peller, Santa Clara, CA.
Stachel, S.E., Messens, E., Van Montagu, M. and Zambryski, P. 1985. Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature 318: 624–629.
Thomas, B.R. and Pratt, D. 1981. Efficient hybridization between Lycopersicon esculentum and L. peruvanum via embryo callus. Theor. Appl. Genet. 59: 215–219.
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Fillatti, J., Kiser, J., Rose, R. et al. Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium Tumefaciens Vector. Nat Biotechnol 5, 726–730 (1987). https://doi.org/10.1038/nbt0787-726
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DOI: https://doi.org/10.1038/nbt0787-726
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