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Genetically Engineered Secretion of Active Human Interferon and a Bacterial Endoglucanase from Aspergillus Nidulans

Bio/Technology volume 5, pages 713–719 (1987)Cite this article

Abstract

Secretion of biologically active human interferon α2 and a bacterial (Cellulomonas fimi) endoglucanase from Aspergillus nidulans is described. The genes encoding these proteins were linked to NH2–terminal fungal secretion signal sequences, synthetic or natural, and expression was regulated by the strong controllable promoters of the A. nidulans alcohol dehydrogenase I gene (alcA) or the A. niger glucoamylase gene. Regulation of the expression and secretion of the human and bacterial proteins in transformants is maintained under the appropriate induction conditions. Selected sequence changes in the original vector constructions produced levels of interferon α2 (before growth optimization) that approach one mg/liter.

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Authors and Affiliations

  1. Allelix Inc., 6850 Goreway Drive, Mississauga, Ontario, L4V 1P1

    David I. Gwynne, Frank P. Buxton, S. A. Williams, S. Garven & R. Wayne Davies

Authors
  1. David I. Gwynne
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  2. Frank P. Buxton
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  3. S. A. Williams
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  4. S. Garven
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  5. R. Wayne Davies
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Gwynne, D., Buxton, F., Williams, S. et al. Genetically Engineered Secretion of Active Human Interferon and a Bacterial Endoglucanase from Aspergillus Nidulans. Nat Biotechnol 5, 713–719 (1987). https://doi.org/10.1038/nbt0787-713

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