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Paring down signaling complexity

A new method for characterizing signaling responses to pairs of agonists predicts how cells react to higher-order combinations of external stimuli.

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Figure 1: Combining pairwise agonist scanning (PAS) with neural-network modeling to capture a complex stimulus landscape.


  1. Miller-Jensen, K., Janes, K.A., Brugge, J.S. & Lauffenburger, D.A. Nature 448, 604–608 (2007).

    Article  CAS  Google Scholar 

  2. Chatterjee, M.S., Purvis, J.E., Brass, L.F. & Diamond, S.L. Nat. Biotechnol. 28, 727–732 (2010).

    Article  CAS  Google Scholar 

  3. Gaudet, S. et al. Mol. Cell. Proteomics 4, 1569–1590 (2005).

    Article  CAS  Google Scholar 

  4. Janes, K.A. et al. Science 310, 1646–1653 (2005).

    Article  CAS  Google Scholar 

  5. Natarajan, M. et al. Nat. Cell Biol. 8, 571–580 (2006).

    Article  CAS  Google Scholar 

  6. Geva-Zatorsky, N. et al. Cell 140, 643–651 (2010).

    Article  CAS  Google Scholar 

  7. Hsueh, R.C. et al. Sci. Signal. 2, ra22 (2009).

    Article  Google Scholar 

  8. Albeck, J.G. et al. Nat. Rev. Mol. Cell Biol. 7, 803–812 (2006).

    Article  CAS  Google Scholar 

  9. Krogh, A. Nat. Biotechnol. 26, 195–197 (2008).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Kevin A Janes.

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Janes, K. Paring down signaling complexity. Nat Biotechnol 28, 681–682 (2010).

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