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A nuclease-resistant protein kinase Cα ribozyme blocks glioma cell growth

Nature Biotechnology volume 16, pages 556–561 (1998)Cite this article

Abstract

We investigated the cleavage activity, stability, and efficacy of 2′-amino pyrimidine modified ribozymes on malignant glioma growth. A synthetic protein kinase Cα (PKCα) ribozyme with complete pyrimidine nucleotide substitution retained a comparable cleavage activity compared with the unmodified ribozyme. The half-life of the modified ribozyme in serum was increased 14,000-fold compared with the unmodified version. The PKCα modified ribozyme inhibited glioma cell growth in vitro as a result of the inhibition of PKCα gene expression. A single injection of cationic liposome ribozyme complexes into glioma tumors inhibited tumor growth, demonstrating both the efficacy of the ribozyme and a major role of PKCα in tumor growth.

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Authors and Affiliations

  1. Institute for Cancer Research, Department of Immunology, The Norwegian Radium Hospital, Montebello, N-0310, Oslo

    Mouldy Sioud

  2. Animal Unit, The National Hospital, N-0027, Oslo, Norway

    Dag R. Sørensen

Authors
  1. Mouldy Sioud
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  2. Dag R. Sørensen
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Correspondence to Mouldy Sioud.

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Sioud, M., Sørensen, D. A nuclease-resistant protein kinase Cα ribozyme blocks glioma cell growth. Nat Biotechnol 16, 556–561 (1998). https://doi.org/10.1038/nbt0698-556

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  • DOI: https://doi.org/10.1038/nbt0698-556

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