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Enzyme Immobilization Using the Cellulose-Binding Domain of a Cellulomonas Fimi Exoglucanase

Bio/Technology volume 7, pages 604–607 (1989)Cite this article

Abstract

We have constructed a fusion between the gene (abg) for a β-glucosidase (Abg) of an Agrobacterium sp. and part of the gene (cex) for an exoglucanase (Cex) of Cellulomonas fimi. The abg-cex fusion encodes a fusion protein (Abg-CBDCex) with the cellulose-binding properties of Cex and β-glucosidase activity of Abg. The fusion protein retained 42 percent of its β-glucosidase activity when bound to cellulose. The binding was stable enough to allow continuous hydrolysis of substrate by the enzyme without detectable leaching of the enzyme from the cellulose. We believe that the cellulose-binding domain (CBDCex) of Cex offers a simple, inexpensive and widely applicable method of enzyme immobilization.

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Author notes

  1. Douglas G. Kilburn: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Microbiology, University of British Columbia, 300-6174 University Boulevard, Vancouver, B.C., Canada, V6T 1W5

    Edgar Ong, Neil R. Gilkes, R. Antony J. Warren, Robert C. Miller Jr. & Douglas G. Kilburn

Authors
  1. Edgar Ong
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  2. Neil R. Gilkes
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  3. R. Antony J. Warren
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  4. Robert C. Miller Jr.
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  5. Douglas G. Kilburn
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Ong, E., Gilkes, N., Warren, R. et al. Enzyme Immobilization Using the Cellulose-Binding Domain of a Cellulomonas Fimi Exoglucanase. Nat Biotechnol 7, 604–607 (1989). https://doi.org/10.1038/nbt0689-604

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