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Production and Characterization of Growth Hormone Releasing Factor Analogs Through Recombinant DNA and Chemical Techniques

Bio/Technology volume 4, pages 565–568 (1986)Cite this article

Abstract

A growth hormone releasing factor analog [Leu27,Hse44]GRF(1–44)lactone has been made by recombinant DNA techniques. The peptide was expressed from a multiple–copied GRF gene fused to lacZ. The monomeric GRF peptide analog with a C–terminal homoserine lactone was obtained upon treatment of the isolated fusion protein with cyanogen bromide. The yield of the analog increased with the number of copies fused to β–galactosidase. The C–terminal homoserine moiety was chemically converted to the primary amide, [Leu27,Hse44]GRF(1–44)NH2 and also the n–butyl and n–dodecyl amides, [Leu27, Hse44]GRF(1–44)NHC4H9 and [Leu27,Hse44]GRF(1–44)NHC12H25. The primary amide exhibited a slightly higher releasing activity than the naturally occurring hpGRF(1–44)NH2 whereas the secondary amide analogs had lower activities.

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Author notes

  1. Pamela Baker: American Cyanamid Company, Princeton, NJ 08540.

Authors and Affiliations

  1. Molecular Genetics, Inc., Minnetonka, MN, 55343

    Tomas Kempe, Flora Chow, Susan M. Peterson, William Hays, Gary Opperman, James J. L'Italien, Gary Long & Brad Paulson

Authors
  1. Tomas Kempe
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  2. Flora Chow
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  3. Susan M. Peterson
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  4. Pamela Baker
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  5. William Hays
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  6. Gary Opperman
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  7. James J. L'Italien
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  8. Gary Long
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  9. Brad Paulson
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Kempe, T., Chow, F., Peterson, S. et al. Production and Characterization of Growth Hormone Releasing Factor Analogs Through Recombinant DNA and Chemical Techniques. Nat Biotechnol 4, 565–568 (1986). https://doi.org/10.1038/nbt0686-565

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