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STEM Footprints and Bound Mass Distributions for DNA Control Proteins

Bio/Technology volume 3, pages 549–553 (1985)Cite this article

Abstract

We review a current methodology which analyzes quantitative scattering data from Scanning Transmission Electron Microscopy to establish footprints—positions of DNA-binding proteins on cloned genes—and mass distributions for the bound protein. Recognition by computer of the DNA path and the extent of the bound protein leads to an analysis rate which is high for image-based data. The methods have been applied in the study of eukaryotic transcription initiation and T antigen interaction with the SV40 control region.

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Author notes

  1. Iris A. Mastrangelo, Van G. Wilson, Kevin Ryder and Peter Tegtmeyer: State University of New York, Stony Brook, NY 11794

Authors and Affiliations

  1. Biology Department, Brookhaven National Laboratory, Upton, NY, 11973

    Paul V. C. Hough, Joseph S. Wall & James F. Hainfeld

Authors
  1. Paul V. C. Hough
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  2. Iris A. Mastrangelo
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  3. Joseph S. Wall
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  5. Van G. Wilson
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  7. Peter Tegtmeyer
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Hough, P., Mastrangelo, I., Wall, J. et al. STEM Footprints and Bound Mass Distributions for DNA Control Proteins. Nat Biotechnol 3, 549–553 (1985). https://doi.org/10.1038/nbt0685-549

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