Abstract
To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1–5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.
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Ikeno, M., Grimes, B., Okazaki, T. et al. Construction of YAC–based mammalian artificial chromosomes. Nat Biotechnol 16, 431–439 (1998). https://doi.org/10.1038/nbt0598-431
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DOI: https://doi.org/10.1038/nbt0598-431
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