Research Paper | Published:

Piezoelectric Cell Growth Sensor

Bio/Technology volume 9, pages 450454 (1991) | Download Citation

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Abstract

We have developed a reusable piezoelectric sensor that enables rapid characterization of cell viability and response to cell-affecting agents. This is accomplished via a novel polymer transduction principle that involves reaction of a pH-sensitive amphoteric polymer with metabolically generated acid. Subsequent adhesion of the protonated polymer to the transducer surface causes a decrease in the sensor resonant frequency corresponding to the cell metabolic rate. This disclosure provides the first example of a piezoelectric sensor capable of detecting metabolic responses of viable cells. The sensor provides real-time measurement of cell metabolism and division rates, and antibiotic sensitivity. This technology provides the basis for an advanced piezoelectric sensor that does not require immobilized biological receptors and can be miniaturized without compromising signal-to-noise factors.

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References

  1. 1.

    1985. Rapid Methods and Automation in Microbiology and Immunology, Springer-Verlag, Berlin.

  2. 2.

    1988. The Viable Count: Quantitative and Environmental Aspects, Brock-Springer, Madison.

  3. 3.

    1986. Detection technology: The key to environmental biotechnology. Bio/Technology 4: 419–422.

  4. 4.

    1985. Instrumental Methods for Rapid Microbiological Analysis, VCH, Wienheim.

  5. 5.

    1987. Analytical device and method for analysis of biochemicals, microbes and cells. European Patent Application No. 86307115.5.

  6. 6.

    and 1990. In-situ interfacial mass detection with piezoelectric transducers: Fundamentals and applications. Science In press

  7. 7.

    1959. Verwendung von Schwingquarzen zur Wagung dunner Schichten und zur Mikrowagung. Z. Phys. 155: 206–222.

  8. 8.

    , , , , , , , , and 1989. Detection of cell-affecting agents with a silicon biosensor. Science 246: 243–247.

  9. 9.

    1988. Acrylic amphoteric polymers. U.S. Patent 4,749,762.

  10. 10.

    1987. Escherichia Coli and Salmonella Typhimurium Cellular and Molecular Biology. Neidhardt, F. C., Ingraham, J. L., Brooks, K., Magasanik, M., Schaechter, M., and Umbarger, H. E. (Eds.). American Society for Microbiology, Washington, D.C.

  11. 11.

    1987. Bacterial Metabolism, Verlag-Springer, New York.

  12. 12.

    and 1989. Sensors based on biomolecules immobilized on the piezoelectric quartz crystal microbalance . Chemical Sensors and Microinstrumentation 403: 237–251.

  13. 13.

    , and 1989. Detection of odorants using lipid-coated piezoelectric crystal resonators. Anal. Chim. Acta 225: 399–408.

  14. 14.

    , and 1986. Correlation of surface acoustic wave device coating responses with solubility properties and chemical structure using pattern recognition. Anal. Chem. 58: 3058–3066.

  15. 15.

    , , , and 1986. Selection of adsorbates for chemical sensor arrays by pattern recognition. Anal. Chem. 58: 149–153.

  16. 16.

    , , and 1989. Odour-sensing system using a quartz-resonator sensor array and neural-network pattern recognition. Sensors and Actuators 18: 291–296.

  17. 17.

    and 1988. A multisensor employing an ultrasonic Lamb-wave oscillator. IEEE Trans. Elect. Dev. 35: 735–743.

  18. 18.

    , , and 1989. Liquid-phase sensors based on acoustic plate mode devices. Chemical Sensors and Microinstrumentation 403: 191–207.

  19. 19.

    1988. Investigation of open circuit reactions of polymer films using the quartz crystal microbalance. Reactions of polyvinylfer-rocene films. J. Phys. Chem. 92: 2049–2054.

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Author information

Author notes

    • Michael D. Ward

    Present address: Chemical Engineering and Material Science Department, University of Minnesota, Amundson Hall, 421 Washington Ave. SE, Minneapolis, MN 55455.

    • Richard C. Ebersole
    •  & Michael D. Ward

    Corresponding authors.

Affiliations

  1. Central Research and Development Department, E.I. du Pont de Nemours & Co., Experimental Station, P.O. Box 80173, Wilmington, DE 19880-0173.

    • Richard C. Ebersole
    •  & Robert P. Foss

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Publication history

Received

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Published

DOI

https://doi.org/10.1038/nbt0591-450

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