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Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion

Bio/Technology volume 8, pages 435–440 (1990)Cite this article

Abstract

We have extended the work on chymosin production in Aspergillus by constructing an expression vector in which the cDNA encoding bovine prochymosin B was fused in frame immediately following the codon for the last amino acid of the A. awamori glucoamylase (glaA) gene. Transformation of A. awamori with this plasmid led to the secretion of considerably higher amounts of chymosin than obtained with previous chymosin expression vectors. We present evidence that mature chymosin is autocatalytically released from the glucoamylase-chymosin fusion protein after secretion.

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Authors and Affiliations

  1. Genencor Inc., 180 Kimball Way, South San Francisco, CA, 94080

    Michael Ward, Lori J. Wilson, Katherine H. Kodama, Michael W. Rey & Randy M. Berka

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  1. Michael Ward
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  3. Katherine H. Kodama
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  4. Michael W. Rey
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  5. Randy M. Berka
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Ward, M., Wilson, L., Kodama, K. et al. Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion. Nat Biotechnol 8, 435–440 (1990). https://doi.org/10.1038/nbt0590-435

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