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Notes on Culture Media

Bio/Technology volume 6, pages 575–581 (1988)Cite this article

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References

  1. Ozarto, K., Mayer, N. and Sachs, D.H. 1980. Hybridoma Cell Lines Secreting Monoclonal Antibodies to Mouse H-2 and Ia Antigens. J. Immunology. 124:533–540.

    Google Scholar 

  2. Long, W.J., Palombo, A., Schofield, T.L., and Eminie, E.A. 1988. Effects of Culture Media on Murine Hybridomas: Definition of Optimal Conditions for Hybridoma Viability, Cellular Proliferation, and Antibody Production. Hybridoma. 7:69–77.

    Article  CAS  Google Scholar 

  3. Iscove, N.N. and Melchers, F. 1978. Complete Replacement of Serum by Albumin, Transferrin, and Soybean Lipid in Cultures of Lipopolysaccharide-Reactive B Lymphocytes. J. Exp. Med. 147:923–933.

    Article  CAS  Google Scholar 

  4. Cleveland, W.L., Wood, I., and Erlanger, B.F. 1983. Routine Large-Scale Production of Monoclonal Antibodies in a Protein-Free Culture Medium. J. Imm. Meth. 56:221–234.

    Article  CAS  Google Scholar 

  5. Antoniades, H.N., Scher, C.D. and Stiles, C.D. 1979. Purification of Human Platelet-Derived Growth Factor. Proc. Natl. Acad. Sci. USA 76:1809–1813.

    Article  CAS  Google Scholar 

  6. Helden, C.-H., Westermark, B. and Wasteson, A. 1979. Platelet-Derived Growth Factor: Purification and Partial Characterization. Proc. Natl. Acad. Sci. USA 76:3722–3726.

    Article  Google Scholar 

  7. Maciage, T., Kelly, B., Cerundolo, J., Ilsley, S., Kelley, P.R., Gaudreau, J., and Forand, R. 1980. Hormonal Requirements of Baby Hamster Kidney Cells in Culture. Cell. Biol. Int. Rep. 4:43–50.

    Article  Google Scholar 

  8. McKeehan, W.L. and Ham, R.G. 1977. Methods for Reducing the Serum Requirement for Growth in vitro of Nontransformed Diploid Fibroblasts. Dev. Biol. Stand. 37:97–108.

    CAS  Google Scholar 

  9. Hutchings, S.F. and Sato, G.H. 1978. Growth and Maintenance of Hela Cells in Serum-Free Medium Supplemented with Hormones. Proc. Natl. Acad. Sci. USA 79:4074–4077.

    Google Scholar 

  10. Wolfe, R.A., Braatz, J.A., Miller, D.A., and Heifetz, A.H. 1988. A New Serum-Free Medium for Monoclonal Antibody Production. Biotechniques. 6:62–67.

    CAS  PubMed  Google Scholar 

  11. Glassy, M.C., Peters, R.E., and Mikhalev, A. 1987. Growth of Human-Human Hybridomas in Serum-Free Media Enhances Antibody Secretion. In Vitro Cell. & Dev. Biol. 23:745–749.

    Article  CAS  Google Scholar 

  12. Higuchi, K. 1977. Cultivation of Mammalian Cell Lines in Serum-Free Chemically Defined Medium. Cell Biol. 14:131–136.

    Google Scholar 

  13. Barnes, D., and Sato, G. 1980. Serum-Free Cell Culture: A Unifying Approach. Cell. 22:649–655.

    Article  CAS  Google Scholar 

  14. Stampfer, M.R., Hackett, A.J., Smith, H.S., Hancock, M.C., Leung, J.P., and Edginton, T.S. 1982. Cold Spring Harbor Conf. Cell Proliferation. 9:819–829.

  15. Hammond, S.L., Ham, R.G., and Stampfer, M.R. 1984. Serum-Free Growth of Human Mammary Epithelial Cells: Rapid Clonal Growth in Defined Medium and Extended Serial Passage with Pituitary Extract. Proc. Natl. Acad. Sci. USA 81:5435–5439.

    Article  CAS  Google Scholar 

Download references

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  1. editor of Telegen, an electronic biotechnology database published by EIC Intelligence, 48 West 38th Street, New York, NY, 10018

    Jane Williams

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Williams, J. Notes on Culture Media. Nat Biotechnol 6, 575–581 (1988). https://doi.org/10.1038/nbt0588-575

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