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Crystallization of a Lignin Peroxidase from the White–Rot Fungus Phanerochaete Chrysosporium

Bio/Technology volume 6, pages 571–573 (1988)Cite this article

An Erratum to this article was published on 01 July 1988

Abstract

The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P212121 and four lignin peroxidase molecules per unit cell.

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Author notes

  1. Jan Derk G. Smit and Kaspar. H. Winterhalter: Laboratory of Biochemistry, Swiss Federal Institute of Technology, 8000 Zürich, Switzerland.

  2. Jörg Kallen: Department of Structural Biology, Biozentrum, 4056 Basel, Switzerland.

  3. Jan Derk G. Smit: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Biotechnology, Swiss Federal Institute of Technology, 8000, Zürich, Switzerland

    Jakob Troller, Matti S. A. Leisola & Armin Fiechter

Authors
  1. Jakob Troller
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  2. Jan Derk G. Smit
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  3. Matti S. A. Leisola
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  4. Jörg Kallen
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  5. Kaspar. H. Winterhalter
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  6. Armin Fiechter
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Troller, J., Smit, J., Leisola, M. et al. Crystallization of a Lignin Peroxidase from the White–Rot Fungus Phanerochaete Chrysosporium. Nat Biotechnol 6, 571–573 (1988). https://doi.org/10.1038/nbt0588-571

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  • DOI: https://doi.org/10.1038/nbt0588-571

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