The Structure and Bacterial Expression of Three Distinct Bovine Interferon-β Genes


Three bovine interferon-β (BoIFN-β) genes were identified in a bacteriophage λ/bovine genomic library by hybridization to a human IFN-β cDNA probe. DNA sequence analysis demonstrates that these genes contain no introns and code for homologous, yet distinct, proteins. Expression in Escherichia coli of each of the three BoIFN-β genes results in the synthesis of biologically active IFN-β. Southern hybridization analysis indicates the presence of at least five members in the bovine IFN-β gene family.

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Leung, D., Capon, D. & Goeddel, D. The Structure and Bacterial Expression of Three Distinct Bovine Interferon-β Genes. Nat Biotechnol 2, 458–464 (1984).

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