Addendum | Published:

Addendum: Chinese hamster ovary cells can produce galactose-α-1, 3-galactose antigens on proteins

Nature Biotechnology volume 29, page 459 (2011) | Download Citation

Nat. Biotechnol. 28, 1153–1156 (2010); published online 5 November 2010; addendum published after print 12 April 2011

Over the last 20 years, numerous investigations examining the glycosylation of proteins expressed in Chinese hamster ovary (CHO) cells have failed to document the ability of CHO cells to decorate proteins with galactose-α-1,3-galactose (α-Gal) epitopes1,2,3,4,5,6. However, the ability of CHO cells to produce the α-Gal epitope in recombinant proteins was reported before7. The mechanism of activation of the α-(1,3)-galactosyltransferase in CHO cells remains unclear. A possible factor may involve the transfection process, as similar activation has been documented with other glycosyltransferases8.

References

  1. 1.

    , & Getting the glycosylation right: implications for the biotechnology industry. Nat. Biotechnol. 14, 975–981 (1996).

  2. 2.

    & The Galalpha1,3Galbeta1,4GlcNAc-R (alpha-Gal) epitope: a carbohydrate of unique evolution and clinical relevance. Biochim. Biophys. Acta 1780, 75–88 (2008).

  3. 3.

    , & Characterization of monoclonal antibody glycosylation: comparison of expression systems and identification of terminal alpha-linked galactose. Anal. Biochem. 247, 102–110 (1997).

  4. 4.

    et al. Trends in glycosylation, glycoanalysis and glycoengineering of therapeutic antibodies and Fc-fusion proteins. Curr. Pharm. Biotechnol. 9, 482–501 (2008).

  5. 5.

    , , , & Carbohydrate structures of recombinant soluble human CD4 expressed in Chinese hamster ovary cells. Biochemistry 30, 2395–2406 (1991).

  6. 6.

    et al. Comparative study of the asparagine-linked sugar chains of natural human interferon-beta 1 and recombinant human interferon-beta 1 produced by three different mammalian cells. J. Biol. Chem. 263, 17508–17515 (1988).

  7. 7.

    et al. Site-specific glycosylation of recombinant rat and human soluble CD4 variants expressed in Chinese hamster ovary cells. J. Biol. Chem. 268, 3260–3267 (1993).

  8. 8.

    , , & Transfection of a human alpha-(1,3)fucosyltransferase gene into Chinese hamster ovary cells. Complications arise from activation of endogenous alpha-(1,3)fucosyltransferases. J. Biol. Chem. 265, 1615–1622 (1990).

Download references

About this article

Publication history

Published

DOI

https://doi.org/10.1038/nbt0511-459e

Authors

  1. Search for Carlos J Bosques in:

  2. Search for Brian E Collins in:

  3. Search for James W Meador in:

  4. Search for Hetal Sarvaiya in:

  5. Search for Jennifer L Murphy in:

  6. Search for Guy DelloRusso in:

  7. Search for Dorota A Bulik in:

  8. Search for I-Hsuan Hsu in:

  9. Search for Nathaniel Washburn in:

  10. Search for Sandra F Sipsey in:

  11. Search for James R Myette in:

  12. Search for Rahul Raman in:

  13. Search for Zachary Shriver in:

  14. Search for Ram Sasisekharan in:

  15. Search for Ganesh Venkataraman in: