Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Paper
  • Published:

Human–Human Hybridomas Secreting Hepatitis B Virus–Neutralizing Antibodies

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Lerner, R.A. 1982. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299:592–596.

    Article  CAS  Google Scholar 

  2. Panicali, D. and Paoletti, E. 1982. Construction of poxviruses as cloning vectors: Insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc. Natl. Acad. Sci. USA 79:4927–4931.

    Article  CAS  Google Scholar 

  3. Smith, G.L., Mackett, M. and Moss, B. 1983. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen. Nature 302:490–495.

    Article  CAS  Google Scholar 

  4. McAleer, W.J., Buynak, E.B., Maigetter, R.Z., Wambler, D.E., Miller, W.J. and Hilleman, M.R. 1984. Human hepatitis B vaccine from recombinant yeast. Nature 307:178–180.

    Article  CAS  Google Scholar 

  5. Neurath, A.R., Kent, S.B., Parker, K., Prince, A.M., Strick, N., Brotman, B. and Sproul, P. 1986. Antibodies to a synthetic peptide from the preS 120–145 region of the hepatitis B virus envelope are virus-neutralizing. Vaccine 4:35–37.

    Article  CAS  Google Scholar 

  6. Szmuness, W., Stevens, C.E. and Harley, E.J. 1980. Hepatitis B vaccine: Demonstration of efficacy in a controlled clinical trial in a high-risk population in the United States. N. Engl. J. Med. 303:833–841.

    Article  CAS  Google Scholar 

  7. Seeff, L.B. 1984. Viral Hepatitis and Liver Diseases. Vyas, G. N., Dienstag, J. L. and Hoofnagle, J. H. (Eds.). Grune and Stratton, Florida.

    Google Scholar 

  8. Szmuness, W., Stevens, C.E., Oleszko, W.R. and Goodman, A. 1981. Passive-active immunization against hepatitis B: Immunogenicity studies in adult Americans. Lancet 1:575–577.

    Article  CAS  Google Scholar 

  9. Ichimori, Y., Sasano, K., Ito, H., Hitotsumachi, S., Kimura, Y., Kaneko, K., Kida, M. and Tsukamoto, K. 1985. Establishment of hybridomas secreting human monoclonal antibodies against tetanus toxin and hepatitis B virus surface antigen. Biochem. Biophys. Res. Commun. 129:26–33.

    Article  CAS  Google Scholar 

  10. Ichimori, Y., Harada, K., Hitotsumachi, S. and Tsukamoto, K. 1987. Establishment of hybridoma secreting human monoclonal antibody against hepatitis B surface antigen. Biochem. Biophys. Res. Commun. 142:805–812.

    Article  CAS  Google Scholar 

  11. Prince, A.M., Szmuness, W. and Woods, K.R. 1971. Antibody against serum-hepatitis antigen: Prevalence and potential use as immune serum globulin in prevention of serum-hepatitis infections. N. Engl. J. Med. 285:933.

    Article  CAS  Google Scholar 

  12. Kitano, K., Shintani, Y., Ichimori, Y., Tsukamoto, K., Sasai, S. and Kida, M. 1986. Production of human monoclonal antibodies by heterohybridomas. Appl. Microbiol. Biotechnol. 24:282–286.

    Article  CAS  Google Scholar 

  13. Ito, Y., Hayakawa, T. and Fujisawa, Y. 1986. Expression of hepatitis B virus surface antigen P31 gene in yeast. Biochem. Biophys. Res. Commun. 138:268–274.

    Article  Google Scholar 

  14. Ito, Y. and Fujisawa, Y. 1986. Synthesis in yeast of hepatitis B virus surface antigen modified P31 particles by gene modification. Biochem. Biophys. Res. Commun. 141:942–948.

    Article  Google Scholar 

  15. Kitano, K., Nakao, M., Ito, Y. and Fujisawa, Y. 1987. Recombinant hepatitis B virus surface antigen P31 accumulates as particles in Saccharomyces cerevisiae. Bio/Technology 5:281–283.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harada, K., Ichimori, Y., Sasano, K. et al. Human–Human Hybridomas Secreting Hepatitis B Virus–Neutralizing Antibodies. Nat Biotechnol 7, 374–377 (1989). https://doi.org/10.1038/nbt0489-374

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0489-374

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing