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Recovery of Antigens From Immunoadsorbents Using High Pressure

Bio/Technology volume 7, pages 369–373 (1989)Cite this article

Abstract

We report the use of pressures ranging to 2000 atm to elute bovine serum albumin from a moderate affinity monoclonal antibody immunoadsorbent (K = 2.4 × 107 M−1). Over 75 percent of reversibly bound protein was recovered following a single 15 minute incubation at 2000 atm, and over 90 percent recoveries were obtained by repeated pressurizations. Repeated pressurizations to 2000 atm exerted no detrimental effect on immunoadsorbent binding properties, whereas immunoadsorbent binding capacity was significantly reduced upon treatment with a common chemical eluent, glycine/HCl at pH 2.5.

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Author notes

  1. Martin L. Yarmush: Department of Chemical and Biochemical Engineering, Rutgers University, P.O. Box 909, Piscataway, NJ 08854. To whom reprint requests should be addressed.

Authors and Affiliations

  1. Biotechnology Process Engineering Center, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 66-501, Cambridge, MA, 02139

    William C. Olson, Suzanne K. Leung & Martin L. Yarmush

Authors
  1. William C. Olson
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  2. Suzanne K. Leung
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  3. Martin L. Yarmush
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Olson, W., Leung, S. & Yarmush, M. Recovery of Antigens From Immunoadsorbents Using High Pressure. Nat Biotechnol 7, 369–373 (1989). https://doi.org/10.1038/nbt0489-369

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  • DOI: https://doi.org/10.1038/nbt0489-369

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