Abstract
Homogeneous enzyme membrane immunoassays (EMIAs) have been developed for thyroxine (T4) and human immuno-globulin G (IgG). Liposomes tagged with T4 and containing alkaline phosphatase were used as a model system for optimization of (a) liposome membrane attack in the presence of T4 antiserum and guinea pig complement and (b) subsequent measurement of unmasked enzyme activity. Using a one-incubation-step format, the concentration of T4 in human serum samples was measured. Alkaline phosphatase activity was inversely proportional to the concentration of T4 in the sample. The T4 values obtained by EMIA correlated well with values obtained by radioimmunoassay (r = 0.96). Likewise, there was an excellent correlation between IgG values obtained by EMIA and radialimmuno-diffusion or nephelometry (r = 0.99).
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Braman, J., Broeze, R., Bowden, D. et al. Enzyme Membrane Immunoassay (EMIA). Nat Biotechnol 2, 349–355 (1984). https://doi.org/10.1038/nbt0484-349
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DOI: https://doi.org/10.1038/nbt0484-349