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The potential of nucleic acid repair in functional genomics

Nature Biotechnology volume 19, pages 321–326 (2001)Cite this article

Abstract

Chimeric RNA/DNA oligonucleotides have been used successfully to correct point and frameshift mutations in cells as well as in animal and plant models. This approach is one of several nucleic acid repair technologies that will help elucidate the function of newly discovered genes. Understanding the mechanisms by which these different technologies direct gene alteration is essential for progress in their application to functional genomics.

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Figure 1: Simplified scheme of different approaches for gene targeting and correction at the RNA level.

© Bob Crimi

Figure 2: Simplified scheme of different approaches for gene targeting and correction at the DNA level.

© Bob Crimi

Figure 3: Schematic representation of the constructs used in the yeast assay system.
Figure 4: Experimental assay system used to test gene repair in live Saccharomyces cerevisiae cells.

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Acknowledgements

We thank our many colleagues for providing us with information regarding their work, and we apologize for any omissions due to space limitations. This work was supported in part by grants from the National Institutes of Health. Special thanks go to Eric M. Roberts for his outstanding graphic and technical skills during manuscript preparation.

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  1. Department of Biological Science, University of Delaware, Newark, 19716, DE

    Michael C. Rice, Kirk Czymmek & Eric B. Kmiec

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  1. Michael C. Rice
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Correspondence to Eric B. Kmiec.

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Rice, M., Czymmek, K. & Kmiec, E. The potential of nucleic acid repair in functional genomics. Nat Biotechnol 19, 321–326 (2001). https://doi.org/10.1038/86701

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