Abstract
Soybean (Glycine max cv. “Forrest”) has been successfully transformed with Agrobacterium containing a bacterial kanamycin resistance gene linked to the 5′ portion of a soybean small subunit carboxylase gene. Comparison of light-grown versus dark-grown transformed callus demonstrated light induction of the chimeric gene as measured by increased levels of kanamycin resistance. This is not unexpected as small subunit genes are under light regulation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Herrera-Estrella, L., Depicker, A., Van Montague, M. and Schell, J. 1983. Expression of chimaeric genes transferred into plant cells using a Ti- plasmid-derived vector. Nature 303: 209–213.
Herrera-Estrella, L., De Block, M., Messens, E., Hernalsteens, J.-P., Van Montague, M. and Schell, J. 1983. Chimeric genes as dominant selectable markers in plant cells. EMBO J. 2: 987–995.
Fraley, R., Rogers, S., Horsch, R., Sanders, P., Flick, J., Adams, S., Bittner, M., Brand, L., Fink, F., Fry, J., Gallupi, G., Goldberg, S., Hoffman, N. and Wood, S. 1983. Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80: 4803–4807.
Bevan, M., Flavell, R. and Chilton, M.-D. 1983. A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184–187.
Horsch, R.B., Fraley, R.T., Rogers, S.G., Sanders, P.R., Lloyd, A. and Hoffman, N. 1984. Inheritance of functional foreign genes in plants. Science 223: 496–498.
DeBlock, M., Herrera-Estrella, L., Van Montagu, M., Schell, J. and Zymbryski, P. 1984. Expression of foreign genes in regenerated plants and their progeny. EMBO J. 3: 1681–1689.
Tobin, E.M. 1975. Light regulation of specific mRNA species in Lemna gibb L. G-30. Proc. Natl. Acad. Sci. USA 75: 4749–4753.
Bedbrook, J.R., Smith, S.M. and Ellis, R.J. 1980. Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-l,5-bisphosphate carboxylase. Nature 287: 692–697.
Smith, S.M. and Ellis, R.J. 1981. Light-stimulated accumulation of transcripts of nuclear and chloroplast genes for ribulosebisphosphate carboxylase. J. Mol. Appl. Gen. 1: 127–137.
Stiekma, W.J., Wimpee, C.F., Siverthorne, J. and Tobin, E. 1983. Phytochrome control of the expression of two nuclear genes encoding chloroplast proteins in Lemna gibba L. G-3. Plant Physiol. 72: 717–724.
Berry-Lowe, S., McKnight, T., Shah, D. and Meagher, R. 1982. The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-l,5-bisphos-phate carboxylase in soybean. J. Mol. Appl. Gen. 1: 483–498.
Herrera-Estrella, L., Van den Broeck, G., Maenhaut, R., Van Montague, M., Schell, J., Timko, M. and Cashmore, A. 1984. Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature 310: 115–120.
DeCleene, M. and DeLey, J. 1976. The host range of crown gall. Botan. Rev. 42: 389–466.
Pedersen, H., Christiansen, J. and Wyndalle, R. 1983. Induction and in vitro culture of soybean crown gall tumors. Plant Cell Repts. 2: 201–204.
Hood, E.E., Jen, G., Kayes, L., Kramer, J., Fraley, R.T. and Chilton, M.-D., 1984. Restriction Endonuclease map of pTi Bo542, a potential Ti plasmid vector for genetic engineering of plants. Bio/Technology 2: 702–708.
Christianson, M.L., Warnick, D.A. and Carlson, P.S. 1983. A morphogenetically competent soybean suspension culture.Science 222: 632–634.
Shewmaker, C., Caton, J., Houck, C. and Gardner, R. 1985. Transcription of cauliflower mosaic virus integrated into plant genomes. Virology (in press).
Koncz, C., De Grene, H., Andre, D., DeBoeck, F., Van Montague, M. and Schell, J. 1983. The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants. EMBO J. 2: 1597–1602.
Haas, M. and Dowding, J. 1975. Aminoglycoside-modifying enzymes. Methods in Enzymology 43: 611–628.
Oka, A., Sugisuki, H. and Takami, M. 1981. Nucleotide sequence of the kanamycin resistance transposon in Tn903. J. Biol. Chem. 147: 217–226.
Beck, E., Ludwig, G., Auerswald, E.A., Reiss, B. and Schaller, H. 1982. Nucleotide sequence and exact location of the neomycin phosphotransferase gene from transposon Tn5. Gene 19: 327–336.
Comai, L., Schilling-Cordaro, C., Mergia, A. and Houck, C. 1983. A new technique for genetic engineering of Agrobacterium Ti plasmid. Plasmid 10: 21–30.
Fehr, W.R. and Caviness, C.E. 1977. Stages of soybean development. Iowa Stage Coop. Ext. Serv., Agric. and Home Econ. Expt. Stn. Special Report 80.
Reiss, B., Sprengl, R., Will, H. and Schaller, H. 1984. A new sensitive method for qualitative and quantitative assay for neomycin phosphotransferase in crude cell extracts. Gene 30: 211–218.
Akiyoshi, D., Morris, R., Hinz, R., Mischike, B., Kosuge, T., Garfinkel, D., Gordon, M. and Nester, E. 1983. Cytokinin auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc. Natl. Acad. Sci. USA 80: 407–411.
Garfinkel, D.J., Simpson, R.B., Ream, L.W., White, F.F., Gordon, M.P. and Nester, E.W. 1981. Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesis. Cell 27: 143–153.
Meselson, M. and Yuan, R. 1968. DNA restriction enzyme from E.coli. Nature 217: 1110–1114.
Messing, J., Groenenborn, B., Muller-Hill, B.M. and Hofschneider, P. 1977. Filamentous coliphage M13 as a cloning vehicle: Insertion of a HindII fragment of the lac regulatory region in M13 replication form in vitro. Proc. Natl. Acad. Sci. USA 74: 3642–3646.
Garfinkel, D.J. and Nester, E.W. 1980. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J. Bacteriol. 144: 732–743.
Boyer, H. and Roulland-Dussoix, D. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41: 459.
Ditta, G., Stanfield, S., Corbin, D. and Helinski, D. 1980. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 76: 1648–1652.
Thomashow, M., Nutter, R., Montoya, A., Gordon, M. and Nester, E. 1980. Integration and organization of the Ti plasmid sequences in crown gall tumors. Cell 19: 729–739.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. and Green, M.R., 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage sp6 promoter. Nuc. Acids Res. 12: 7035–7056.
Miller, J. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Chilton, M.-D., Currier, T.C., Farrand, S.K., Bendich, A.J., Gordon, M.P. and Nester, E.W. 1974. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci. USA 71: 3672–3676.
Roberts, W. and Kerr, A. 1974. Crown gall induction: Serological reactions, isozyme patterns and sensitivity to mitomycin C and to bacteriocin of pathogenic and non-pathogenic strains of Agrobacterium radiobacter. Physiol. Plant Pathol. 4: 81–91.
Otten, L. and Schilperoort, R.A. 1978. A rapid micro-scale method for the detection of lysopine and nopaline dehydrogenase activities.Biochem. et Biophys. Acta 527: 497–500.
Maniatis, J., Fritsch, E.J. and Sambrook, J. 1982. Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York.
Currier, T. and Nester, E. 1976. Evidence for diverse types of large plasmids in tumor inducing strains of Agrobacterium. J. Bacteriol. 126: 157–165.
Stalker, D., Hiatt, W. and Comai, L. 1985. A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J. Biol. Chem. (in press).
Colbert, J., Hershey, H. and Quail, P. 1983. Autoregulatory control of translatable phytochrome mRNA levels. Proc. Natl. Acad. Sci. USA 80: 2248–2252.
Schreier, P., Seftor, E., Schell, J. and Bohnert, H.J. 1984. The use of nuclear encoded sequences in direct light regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J. (in press).
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Facciotti, D., O'Neal, J., Lee, S. et al. Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium. Nat Biotechnol 3, 241–246 (1985). https://doi.org/10.1038/nbt0385-241
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt0385-241
This article is cited by
-
A simple and efficient method for obtaining transgenic soybean callus tissues
Acta Physiologiae Plantarum (2013)