Research Paper | Published:

Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium

Bio/Technology volume 3, pages 241246 (1985) | Download Citation

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Abstract

Soybean (Glycine max cv. “Forrest”) has been successfully transformed with Agrobacterium containing a bacterial kanamycin resistance gene linked to the 5′ portion of a soybean small subunit carboxylase gene. Comparison of light-grown versus dark-grown transformed callus demonstrated light induction of the chimeric gene as measured by increased levels of kanamycin resistance. This is not unexpected as small subunit genes are under light regulation.

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Affiliations

  1. Calgene, Inc., 1920 Fifth Street, Davis, CA 95616

    • D. Facciotti
    • , J. K. O'Neal
    • , S. Lee
    •  & C. K. Shewmaker

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DOI

https://doi.org/10.1038/nbt0385-241

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