Review Article | Published:

A Second Role for Second Messengers: Uncovering the Utility of Cyclic Nucleotide Hydrolysis

Bio/Technology volume 3, pages 235238 (1985) | Download Citation

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Abstract

A new technology based on enzyme-catalyzed incorporation of 18O from [18O]water into nucleotide phosphoryls is described that provides the capability to monitor the dynamics of cyclic AMP and cyclic GMP metabolism as well as the metabolism of high energy phosphates in intact cells. This 18O-labeling approach has provided evidence that cyclic GMP hydrolysis, tightly coupled to its synthesis, and independent of changes in cyclic nucleotide steady state levels, may represent a biochemical event of functional utility in its own right, and an essential biochemical event in phototransduction.

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Affiliations

  1. Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455

    • Nelson D. Goldberg
    •  & Timothy F. Walseth

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DOI

https://doi.org/10.1038/nbt0385-235

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