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Mixed Lactic Acid–Alcoholic Fermentation by Saccharomyes cerevisiae Expressing the Lactobacillus casei L(+)–LDH


We describe the construction of a Saccharomyces cerevisiae strain expressing the gene encoding the L(+)–lactate dehydrogenase [L(+)–LDH)] from Lactobacillus casei. The recombinant strain is able to perform a mixed lactic acid–alcoholic fermenation. Yeast cells expressing the L(+)–LDH gene from the yeast alcohol dehydrogenase (ADH1) promoter on a multicopy plasmid simultaneously convert glucose to both ethanol and lactate, with up to 20% of the glucose transformed into L(+)–lactate. Such strains may be used in every field where both biological acidification and alcoholic fermentation are required.

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Dequin, S., Barre, P. Mixed Lactic Acid–Alcoholic Fermentation by Saccharomyes cerevisiae Expressing the Lactobacillus casei L(+)–LDH. Nat Biotechnol 12, 173–177 (1994).

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