Genotyping of Bovine Kappa-Casein Loci Following DNA Sequence Amplification

  • An Erratum to this article was published on 01 August 1990

Abstract

Caseins are a family of milk proteins that exist in several molecular forms (alphas1, alphas2, beta and kappa) with variant alleles of each. Kappa-caseins (κ-CN) exist as A or B variants and are of particular importance to the quality of the milk. We report a rapid non-radioactive method for differentiating bovine k-CN alleles. Unlike direct milk protein analysis, this method can be utilized for genotyping sperm, or animals of either sex at an early age. A 350bp fragment of the bovine κ-CN gene was amplified by the polymerase chain reaction (PCR) technique. The amplified product digested with Hinfl endonuclease generated restriction fragment length polymorphisms (RFLP's) that allowed precise identification of κ-CN AA, AB or BB genotypes. Hybridization with allele specific oligo-probes confirmed the RFLP identification of genotypes. The accurate and early identification of milk protein genotypes can have a direct impact on dairy cattle breeding strategies.

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Medrano, J., Cordova, E. Genotyping of Bovine Kappa-Casein Loci Following DNA Sequence Amplification. Nat Biotechnol 8, 144–146 (1990). https://doi.org/10.1038/nbt0290-144

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