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Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter

Bio/Technology volume 7, pages 169–174 (1989)Cite this article

Abstract

We have constructed an expression vector for the filamentous fungus Aspergillus nidulans using the well characterized amdS promoter system, and tested its potential as an expression system for foreign proteins using the Escherichia coli heat labile enterotoxin subunit B (LTB) gene. Co–transformation with a selectable plasmid was used to introduce an LTB construct into A. nidulans and transformants containing multiple copies of the construct were identified. DNA and mRNA coding for LTB were detected in transformants, and mRNA levels were inducible by acetate via the amdI9 mutation in the expression vector. Mature LTB was detected in cellular extracts but not in the medium, and expression levels correlated with the number of LTB genes.

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Author notes

  1. Michael J. Hynes: Corresponding author.

Authors and Affiliations

  1. Department of Genetics, University of Melbourne, Parkville, Victoria, 3052, Australia

    Ian F. Turnbull & Michael J. Hynes

  2. Biotechnology Australia Pty. Ltd., 28 Barcoo St., East Roseville, N.S.W., 2069, Australia

    Keith Rand & Neil S. Willetts

Authors
  1. Ian F. Turnbull
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  2. Keith Rand
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  3. Neil S. Willetts
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  4. Michael J. Hynes
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Turnbull, I., Rand, K., Willetts, N. et al. Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter. Nat Biotechnol 7, 169–174 (1989). https://doi.org/10.1038/nbt0289-169

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