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Microenvironment: The Key to Improved Cell Culture Products

Bio/Technology volume 7, pages 143–145 (1989)Cite this article

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References

  1. Karkare, S.B., Phillips, P.G., Burke, D.H., and Dean, R.C. 1985. Continuous production of monoclonal antibodies by chemosta-tic and immobilized hybridoma culture, pp. 127–149. In: Large Scale Mammalian Cell Culture, Academic Press, London.

    Chapter  Google Scholar 

  2. Young, M.W. and Dean, R.C. 1987. Optimization of Mammalian-Cell Bioreactors. Bio/Technology 5:835–837.

    CAS  Google Scholar 

  3. Schlichtung, H. 1955, pp. 84–85. In: Boundary Layer Theory, McGraw-Hill, New York.

    Google Scholar 

  4. Yuan, S.W. 1967. Foundations of Fluid Mechanics., Prentice-Hall. Englewood, NJ.

    Google Scholar 

  5. Grobstein, C. 1975. Developmental role of intercellular matrix, pp. 9–16. In: Extracellular Matrix Influences on Gene Expression, Academic Press, New York.

    Chapter  Google Scholar 

  6. Bissell, M., Hall, G., and Parry, G. 1982. How does the extracellular matrix direct gene expression? J. Theor. Bio. 99:31–68.

    Article  CAS  Google Scholar 

  7. Li, M., Aggler, J., Farson, D.A., Hatier, C., Hassell, J. and Bissell, M. 1987. Influence of reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. PNAS 84:136–140.

    Article  CAS  Google Scholar 

  8. Hynes, R.O. 1981. Fibronectin and its relationship to cellular structure and behavior, pp. 295–334. In: Cell Biology of Extracellular Matrix. E. Hay (ed.). Plenum Press, New York.

    Chapter  Google Scholar 

  9. Hynes, R.O. 1987. Integrins: a family of cell surface receptors. Cell 48:549–554.

    Article  CAS  Google Scholar 

  10. Menko, A.S. and Boettiger, D. 1987. Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentation. Cell 51:51–57.

    Article  CAS  Google Scholar 

  11. Obara, M., Kang, M.S., and Yamada, K.M. 1988. Site-directed mutagenesis of cell-binding domain of human fibronectin: separable, synergistic sites mediate adhesive function. Cell 53:649–657.

    Article  CAS  Google Scholar 

  12. Gebb, C., Hayman, E.♯G., Engvall, E., and Ruoshlahti, E. 1986. Interaction of vitronectin with collagen. J. Biol. Chem. 261:16698–16703.

    CAS  PubMed  Google Scholar 

  13. Spray, D.C., Fujita, M., Saez, J.C., Choi, H., Watanabe, T., Hertzberg, E., Rosenberg, L.C., and Reid, L. 1987. Proteoglycans and glycosaminoglycans induce gap junction synthesis and function in primary liver cultures. J. Cell Biol. 105:541–547.

    Article  CAS  Google Scholar 

  14. Dabora, S.L. and Sheetz, M. 1988. The microtubule-dependent formation of a tu-bulovesicular network with characteristics of the ER from cultured cell extracts. Cell 54:27–35.

    Article  CAS  Google Scholar 

  15. Eisenstein, R.S. and Rosens, J.M. 1988. Both cell substratum regulation and hormonal regulation of milk protein gene expression are exerted primarily at the post-transcriptional level. Mol. and Cell Bio. 8:3183–3190.

    Article  CAS  Google Scholar 

  16. Medina, D. Li, M.L. and Bissell, M. 1987. Casein gene expression in mouse mammary epithelial cell lines: dependence upon extracellular matrix and cell type. Exptl. Cell Res. 172:192–203.

    Article  CAS  Google Scholar 

  17. Ben-Ze'ev, A., Robinson, G., Bucher, N., and Farmer, S. 1988. Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepato-cytes. PNAS 85:2161–2165.

    Article  CAS  Google Scholar 

  18. Schuetz, E.G., Li, D., Omiecinski, C., Muller-Eberhard, I., Kleinman, H.K., Elswick, B., and Guzelian, P.S. 1988. Regulation of gene expression in adult rat hepatocytes on a basement membrane matrix. J. Cell. Physiol. 134:309–323.

    Article  CAS  Google Scholar 

  19. Emerman, J.T. and Pitelka, D.R. 1977. Maintenance of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In Vitro 13:316–328.

    Article  CAS  Google Scholar 

  20. Bisbee, C.A., Machen, T.E., and Bern, H.A. 1979. Mouse mammary epithelial cells on floating collagen gels: transepithelial ion transport and effects of prolactin. PNAS 76:536–540.

    Article  CAS  Google Scholar 

  21. Turley, E.A., Erickson, C.A., and Tucker, R.P. 1985. The retention and ultrastructur-al appearances of various extracellular matrix molecules incorporated into three-dimensional hydrated collagen gels. Dev. Biol. 109:347–369.

    Article  CAS  Google Scholar 

  22. Ruzicka, D. 1986. Vitamin A effects on avian epidermal cultures. Ph.D. dissertation, Univ. of California, Berkeley.

  23. Smith, J.C., Singh, J.P., Lillquist, J.S., Goon, D.S., and Stiles, C.D. 1982. Growth factors adherent to cell substrate are mito-genically active in-situ. Nat. 296:154–156.

    Article  CAS  Google Scholar 

  24. Ruoshlahti, E. and Pierschbacher, M.D. 1987. New perspectives in cell adhestion: RGD and integrins. Sci. 238:491–497.

    Article  Google Scholar 

  25. Yamada, K.M., Kennedy, D.W. and Hayashi, M. 1986, pp. 131–143. In: Fibronectin in cell adhesion, differentiation, and growth.

    Google Scholar 

  26. Hayman, E.G., Pierschbacher, M.D., and Ruoshlahti, E. 1985. Detachment of cells from culture substrate by soluble fibronectin peptides. J. Cell Biol. 100:1948–1954.

    Article  CAS  Google Scholar 

  27. Storch, T.G. and Talley, G.D. 1988. Oxygen concentration regulates the proliferative response of human fibroblasts to serum and growth factors. Exptl. Cell Res. 175:317–325.

    Article  CAS  Google Scholar 

  28. Bailey, K.M., Venkatasubramanian, K., and Karkare, S.B. 1985. Immobilized live cell reactor dynamics following dilution rate shift to growth conditions: cell synchrony effects. Biotech. & Bioeng. 27:1208–1213.

    Article  CAS  Google Scholar 

  29. Karkare, S.B., Venkatasubramanian, K., and Veith, W.R. 1986. Design and operating strategies for immobilized living reactor systems, part 1: biosynthesis of canicidin. Ann. NY Acad. Sci. 469:83–90.

    Article  CAS  Google Scholar 

  30. Karkare, S.B., Burke, D.H., Dean, R.C., LeMontt, J., Souw, P., and Venkatasubramanian, K. 1986. Design and operating strategies for immobilized cell reactor systems, part II: production of hormones by recombinant organisms. Ann. NY Acad. Sci. 469:91–96.

    Article  CAS  Google Scholar 

  31. Kivirikko, K.I. and Myalla, R. 1987. Recent developments in posttranslational modification: intracellular processing. Meth. in Enzymol. 144:96–111.

    Article  CAS  Google Scholar 

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  1. Verax Corp., Etna Rd., Lebanon, NH, 03766

    John N. Vournakis & Peter W. Runstadler

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Vournakis, J., Runstadler, P. Microenvironment: The Key to Improved Cell Culture Products. Nat Biotechnol 7, 143–145 (1989). https://doi.org/10.1038/nbt0289-143

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