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Efficient epitope mapping by bacteriophage λ surface display

Nature Biotechnology volume 15, pages 74–78 (1997)Cite this article

Abstract

A bacteriophage λ surface expression system, λfoo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase I to make random epitope libraries. The libraries were screened by affinity selection using a microtiter plate coated with monoclonal antibodies. Direct DNA sequencing of the selected clones defined two distinct epitope sites consisting of nine and 11 amino-acid residues. Affinity selection of random peptide libraries recovered a number of sequences that were similar to each other but distinct from the galectin-3 sequence. These results demonstrate that a single affinity selection of epitope libraries with antibodies is able to define an epitope determinant as small as nine residues long and is more efficient in epitope mapping than random peptide libraries.

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Author notes

  1. Y. Gi Mikawa

    Present address: The Molecular Sciences Institute, 9894 Genesee Ave., 3rd Floor, La Jolla, CA, 92037

  2. Riaz I. Zuberi & Fu-Tong Liu

    Present address: La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr., San Diego, CA, 92121

Authors and Affiliations

  1. Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA, 92037

    Ichiro Kuwabara, Hiroko Maruyama & Ichi N. Maruyama

  2. Departments of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA, 92037

    Riaz I. Zuberi & Fu-Tong Liu

Authors
  1. Ichiro Kuwabara
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  2. Hiroko Maruyama
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  3. Y. Gi Mikawa
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  4. Riaz I. Zuberi
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  5. Fu-Tong Liu
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  6. Ichi N. Maruyama
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Corresponding author

Correspondence to Ichi N. Maruyama.

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Kuwabara, I., Maruyama, H., Mikawa, Y. et al. Efficient epitope mapping by bacteriophage λ surface display. Nat Biotechnol 15, 74–78 (1997). https://doi.org/10.1038/nbt0197-74

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  • DOI: https://doi.org/10.1038/nbt0197-74

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