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Adenovirus dodecahedron, a new vector for human gene transfer

Nature Biotechnology volume 15, pages 52–56 (1997)Cite this article

Abstract

Recombinant adenovirus is one of most efficient delivery vehicles for gene therapy. However, the initial enthusiasm for the use of recombinant adenovirus for gene therapy has been tempered by strong immune responses that develop to the virus and virus-infected cells. Even though recombinant adeno-viruses are replication-defective, they introduce into the recipient cell, together with the gene of interest, viral genetes that might lead to fortuitous recombination if the recipient is infected by wild-type adenovirus. We propose the use of a dodecahedron made of adenovirus pentons or penton bases as an alternative vector for human gene therapy. The penton is a complex of two oligomeric proteins, a penton base and fiber, involved in the cell attachment, internalization, and liberation of virus into the cytoplasm. The dodecahedron retains many of the advantages of adenovirus for gene transfer such as efficiency of entry, efficient release of DNA from endosomes, and wide range of cell and tissue targets. Because it consists of only one or two adenovirus proteins instead of the 11 contained in an adenovirus virion and it does not contain the viral genome, it is potentially a safer alternative to recombinant adenovirus.

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Authors and Affiliations

  1. Institut de Biologie Structurale, 41 avenue des Martyrs, 38027, Grenoble, France

    Rob W.H. Ruigrok

  2. European Molecular Biology Laboratory, Grenoble Outstation, BP 156, 38042, Grenoble, Cedex 9, France

    Pascal Fender, Evelyne Gout, Sebastien Buffet & Jadwiga Chroboczek

Authors
  1. Pascal Fender
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  2. Rob W.H. Ruigrok
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  3. Evelyne Gout
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  4. Sebastien Buffet
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  5. Jadwiga Chroboczek
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Fender, P., Ruigrok, R., Gout, E. et al. Adenovirus dodecahedron, a new vector for human gene transfer. Nat Biotechnol 15, 52–56 (1997). https://doi.org/10.1038/nbt0197-52

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